Anti-hnRNP H 抗体 (ab10374)
Key features and details
- Rabbit polyclonal to hnRNP H
- Suitable for: WB, IHC-P, IP, ICC/IF, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-hnRNP H antibody
hnRNP H 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to hnRNP H -
由来種
Rabbit -
特異性
The antibody was designed against hnRNPH1 and does not recognize hnRNPH3. The epitope recognized by ab10374 maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187). This product may cross-react with hnRNPH2.
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アプリケーション
適用あり: WB, IHC-P, IP, ICC/IF, IHC-Frmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human hnRNP H aa 400 to the C-terminus (C terminal).
Database link: 3187 -
ポジティブ・コントロール
- IP: HeLa whole cell lysate WB: HeLa, HEK-293T, and NIH/3T3 cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antibodies were affinity purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10374の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/10000 - 1/30000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP | (1) |
Use at 4-10 µg/mg of lysate.
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ICC/IF | (2) |
Use at an assay dependent concentration. PubMed: 20020773
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 24462217
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特記事項 |
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WB
1/10000 - 1/30000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa). |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 4-10 µg/mg of lysate. |
ICC/IF
Use at an assay dependent concentration. PubMed: 20020773 |
IHC-Fr
Use at an assay dependent concentration. PubMed: 24462217 |
ターゲット情報
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機能
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG). -
組織特異性
Expressed ubiquitously. -
配列類似性
Contains 3 RRM (RNA recognition motif) domains. -
ドメイン
Each quasi-RRM repeat bound poly(RG), while only the N-terminal QRRM bound poly(RC) and poly(RU). None of the repeats bound detectable amounts of poly(RA). -
細胞内局在
Nucleus > nucleoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 3187 Human
- Entrez Gene: 59013 Mouse
- Entrez Gene: 140931 Rat
- Omim: 601035 Human
- SwissProt: P31943 Human
- SwissProt: O35737 Mouse
- SwissProt: Q8VHV7 Rat
- Unigene: 604001 Human
see all -
別名
- DKFZp686A15170 antibody
- Heterogeneous nuclear ribonucleoprotein H antibody
- Heterogeneous nuclear ribonucleoprotein H1 (H) antibody
see all
画像
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All lanes : Anti-hnRNP H antibody (ab10374) at 0.2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling hnRNP H with ab10374 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of human hnRNP H by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells.
Lane 1: Rabbit polyclonal to hnRNP H (ab10374) at 0.2 µg/mg lysate current batch
Lane 2: Rabbit polyclonal to hnRNP H (ab10374) at 0.2 µg/mg lysate former batch.
Lane 3: Control IgG
ab10374 was used at 1 ug/mL for Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab10374 は 28 報の論文で使用されています。
- You A et al. TTC22 promotes m6A-mediated WTAP expression and colon cancer metastasis in an RPL4 binding-dependent pattern. Oncogene N/A:N/A (2022). WB ; Human . PubMed: 35798874
- Kirchhof L et al. The G3BP1-UPF1-Associated Long Non-Coding RNA CALA Regulates RNA Turnover in the Cytoplasm. Noncoding RNA 8:N/A (2022). PubMed: 35893232
- Wen C et al. SRSF3 and HNRNPH1 Regulate Radiation-Induced Alternative Splicing of Protein Arginine Methyltransferase 5 in Hepatocellular Carcinoma. Int J Mol Sci 23:N/A (2022). PubMed: 36499164
- Tanu T et al. hnRNPH1-MTR4 complex-mediated regulation of NEAT1v2 stability is critical for IL8 expression. RNA Biol 18:537-547 (2021). PubMed: 34470577
- Takahashi K et al. Heterogenous Nuclear Ribonucleoprotein H1 Promotes Colorectal Cancer Progression through the Stabilization of mRNA of Sphingosine-1-Phosphate Lyase 1. Int J Mol Sci 21:N/A (2020). PubMed: 32630435