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  1. Link

    hnrnp-fh-antibody-1g11-ab10689.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing
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Anti-hnRNP F+H 抗体 [1G11] (ab10689)

  • Datasheet
  • SDS
Reviews (9)Q&A (4)References (17)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP F+H antibody [1G11] (ab10689)

    Key features and details

    • Mouse monoclonal [1G11] to hnRNP F+H
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    こちらの製品もご検討ください

    タンパク質
    Product image
    Recombinant Human Daxx protein (ab131785)
    二次抗体
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    一次抗体
    Product image
    Anti-hnRNP K antibody [EP943Y] (ab52600)

    関連製品

    製品の概要

    • 製品名

      Anti-hnRNP F+H antibody [1G11]
    • 製品の詳細

      Mouse monoclonal [1G11] to hnRNP F+H
    • 由来種

      Mouse
    • 特異性

      Human hnRNP F/H.
    • アプリケーション

      適用あり: IHC-Pmore details
    • 種交差性

      交差種: Human
    • 免疫原

      This information is proprietary to Abcam and/or its suppliers.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 精製度

      Protein A purified
    • 特記事項(精製)

      Purified from supernatant.
    • ポリ/モノ

      モノクローナル
    • クローン名

      1G11
    • ミエローマ

      Sp2/0
    • アイソタイプ

      IgG1
    • 研究分野

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • RNA Processing
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • RNA Processing
      • Splicing
      • Epigenetics and Nuclear Signaling
      • Chromatin Binding Proteins
      • DNA / RNA binding

    関連製品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab10689の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P (2)
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    特記事項
    IHC-P
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    ターゲット情報

    • 関連性

      The hnRNPs belong to a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins. hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties.
    • 参照データベース

      • Entrez Gene: 3185 Human
      • Entrez Gene: 3187 Human
      • Omim: 601035 Human
      • Omim: 601037 Human
      • SwissProt: P31943 Human
      • SwissProt: P52597 Human
      • 別名

        • Heterogeneous nuclear ribonucleoprotein F antibody
        • Heterogeneous nuclear ribonucleoprotein H1 antibody
        • hnRNP F antibody
        • hnRNP H antibody
        • HNRNPF antibody
        • HNRNPH1 antibody
        • HNRPH1 antibody
        • mcs94 1 antibody
        • Nucleolin like protein antibody
        • Nucleolin like protein mcs94 1 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP F+H antibody [1G11] (ab10689)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP F+H antibody [1G11] (ab10689)
        IHC image of ab10689 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10689, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      プロトコール

      • Immunohistochemistry protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (17)

      ab10689 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab10689 は 17 報の論文で使用されています。

      • Chu WK  et al. PKC Regulates YAP Expression through Alternative Splicing of YAP 3'UTR Pre-mRNA by hnRNP F. Int J Mol Sci 22:N/A (2021). PubMed: 33445676
      • Herviou P  et al. hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma. Nat Commun 11:2661 (2020). PubMed: 32461552
      • Li M  et al. LncRNA RP11-670E13.6, interacted with hnRNPH, delays cellular senescence by sponging microRNA-663a in UVB damaged dermal fibroblasts. Aging (Albany NY) 11:5992-6013 (2019). PubMed: 31444317
      • Li W  et al. Long non-coding RNA LINC00672 contributes to p53 protein-mediated gene suppression and promotes endometrial cancer chemosensitivity. J Biol Chem 292:5801-5813 (2017). PubMed: 28232485
      • Li R  et al. Misregulation of Alternative Splicing in a Mouse Model of Rett Syndrome. PLoS Genet 12:e1006129 (2016). PubMed: 27352031
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 13 Abreviews or Q&A

      Western blot abreview for Anti-hnRNP F+H antibody [1G11]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Melanoma cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      30 µg
      Specification
      Melanoma cells
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
      Read More

      Abcam user community

      Verified customer

      投稿 Feb 28 2022

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-hnRNP F+H antibody [1G11]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Brain, Cortex)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH = 6, Citrate
      Permeabilization
      Yes - Tween
      Specification
      Brain, Cortex
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Jun 14 2021

      Immunocytochemistry/ Immunofluorescence abreview for Anti-hnRNP F+H antibody [1G11]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Melanoma cells)
      Permeabilization
      No
      Specification
      Melanoma cells
      Blocking step
      Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: 23°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      投稿 Dec 05 2020

      Immunohistochemistry (Frozen sections) abreview for Anti-hnRNP F+H antibody [1G11]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Epidermis (Skin))
      Permeabilization
      No
      Specification
      Epidermis (Skin)
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      投稿 Nov 06 2020

      Immunocytochemistry abreview for Anti-hnRNP F+H antibody [1G11]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Human Cultured Cells (Melanoma cells)
      Permeabilization
      No
      Specification
      Melanoma cells
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      投稿 Oct 29 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-hnRNP F+H antibody [1G11]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Submucosa (gastrointestinal tract))
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: EDTA
      Permeabilization
      Yes - .1% TritonX-100
      Specification
      Submucosa (gastrointestinal tract)
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Sep 29 2020

      Western blot abreview for Anti-hnRNP F+H antibody [1G11]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - nuclear (Brain)
      Loading amount
      40 µg
      Specification
      Brain
      Gel Running Conditions
      Reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Jul 20 2012

      Question

      We have submitted the Abreview for ab10689. Please activate discount code for us to use. Thanks.

      Read More

      Abcam community

      Verified customer

      Asked on Jul 20 2012

      Answer

      Thank you very much for your review of ab10689.

      I have added the discount code to our internal notes of your review, so the code is active and can now be redeemed for a free primary antibody in our catalog.

      Please let me know if you have any questions or if there is anything else that we can do for you. Have a nice day.

      Read More

      Abcam Scientific Support

      Answered on Jul 20 2012

      Question

      I have decided to proceed and test ab10689 for IP and Western with mouse brain samples. Please send me the discount code at your earliest convenience so that I can place the order. Thanks.

      Read More

      Abcam community

      Verified customer

      Asked on Jul 06 2012

      Answer

      DISCOUNT CODE: ***
      Expiration date: November 7, 2012

      This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab10689 with mouse samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Jul 06 2012

      Question

      Looking for an hnRNP F+H antibody to use with mouse samples.

      Read More

      Abcam community

      Verified customer

      Asked on Jul 06 2012

      Answer

      Thank you for your call today and for your questions.

      Sinceab10689 has not been testedwith mouse samples,I can offer a discount off a future purchase if you buy ab10689 now, test itwith mouse samplesand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be worth 1 free primary antibody.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab10689 with mouse samples. I will then send a discount code. This code must be issued before purchasing ab10689 so please wait for my reply before ordering.

      2. Purchase ab10689 either by phone, fax, or online (www.abcam.com).

      3. Test it with mouse samples.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab10689 turns out to be unsuitable for use with mouse samples, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Jul 06 2012

      1-10 of 13 Abreviews or Q&A

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