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Anti-hnRNP A1 (citrulline R97) 抗体 [EPR20176] - BSA and Azide free (ab271981)

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Dot Blot - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)
  • Immunoprecipitation - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)
  • Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free (ab271981)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20176] to hnRNP A1 (citrulline R97) - BSA and Azide free
  • Suitable for: IP, WB, Dot blot
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free
    hnRNP A1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20176] to hnRNP A1 (citrulline R97) - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    Based on sequence homology we predict that this antibody may react with hnRNP A1L2.

  • Tested Applications & Species

    Application Species
    Dot
    Recombinant fragment
    IP
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK-293T transfected with PADI2 and PADI4, respectively, treated with CaCl2 and Ionomycin, whole cell lysates. IP: HEK-293T transfected with PADI4, treated with CaCl2 and Ionomycin, whole cell lysate.

  • 特記事項

    ab271981 is the carrier-free version of ab208028. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR20176

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Dot
Recombinant fragment
IP
Human
アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
Dot blot
Use at an assay dependent concentration.
特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
Dot blot
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.

  • 配列類似性

    Contains 2 RRM (RNA recognition motif) domains.

  • 翻訳後修飾

    Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
    Sumoylated.

  • 細胞内局在

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
  • Information by UniProt

  • 参照データベース

    • 別名

      • HNRNPA 1 antibody
      • Helix destabilizing protein antibody
      • Helix-destabilizing protein antibody
      • Heterogeneous nuclear ribonucleoprotein A1 antibody
      • Heterogeneous nuclear ribonucleoprotein A1B protein antibody
      • Heterogeneous nuclear ribonucleoprotein B2 protein antibody
      • Heterogeneous nuclear ribonucleoprotein core protein A1 antibody
      • hnRNP A1 antibody
      • hnRNP core protein A1 antibody
      • HNRNPA1 antibody
      • HNRPA1 antibody
      • MGC102835 antibody
      • Nuclear ribonucleoprotein particle A1 protein antibody
      • ROA1_HUMAN antibody
      • Single strand DNA binding protein UP1 antibody
      • Single strand RNA binding protein antibody
      • Single-strand RNA-binding protein antibody
      see all

    画像

    • Dot Blot - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)
      Dot Blot - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)

      Dot blot analysis of hnRNP A1 (citrulline R97) labeled with ab208028 at 1/1000 dilution.

      Lane 1: hnRNP A1 (citrulline R97) peptide.
      Lane 2:hnRNP A1 (citrulline R97) non-citrulline peptide.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

      Blocking and dilution buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208028).
    • Immunoprecipitation - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)
      Immunoprecipitation - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)

      hnRNP A1 (citrulline R97) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate with ab208028 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208028 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate 10 µg (Input). 
      Lane 2: ab208028 IP in HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate. 
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208028 in HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate.

      Exposure time: 10 seconds.

      Blocking and dilution buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208028).

    • Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free (ab271981)
      Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free (ab271981)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab271981 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab271981 は論文での使用が確認できていません。

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