Anti-hnRNP A1 (citrulline R97) 抗体 [EPR20176] (ab208028)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20176] to hnRNP A1 (citrulline R97)
- Suitable for: WB, IP, Dot blot
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-hnRNP A1 (citrulline R97) antibody [EPR20176]
hnRNP A1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR20176] to hnRNP A1 (citrulline R97) -
由来種
Rabbit -
特異性
Based on sequence homology we predict that this antibody may react with hnRNP A1L2.
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アプリケーション
適用あり: WB, IP, Dot blotmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HEK-293T transfected with PADI2 and PADI4, respectively, treated with CaCl2 and Ionomycin, whole cell lysates. IP: HEK-293T transfected with PADI4, treated with CaCl2 and Ionomycin, whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR20176 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab208028の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 38 kDa.
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IP |
1/30.
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Dot blot |
1/1000.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 38 kDa. |
IP
1/30. |
Dot blot
1/1000. |
ターゲット情報
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機能
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. -
配列類似性
Contains 2 RRM (RNA recognition motif) domains. -
翻訳後修飾
Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated. -
細胞内局在
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner. - Information by UniProt
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参照データベース
- Entrez Gene: 3178 Human
- Omim: 164017 Human
- SwissProt: P09651 Human
- Unigene: 546261 Human
- Unigene: 655424 Human
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別名
- HNRNPA 1 antibody
- Helix destabilizing protein antibody
- Helix-destabilizing protein antibody
see all
画像
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Lanes 1-2 & 4 : Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] (ab208028) at 1/5000 dilution
Lane 3 : Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] (ab208028) at 1/1000 dilution
Lanes 1 & 3 : Empty vector (vector control) transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate transfected with GFP-tagged PADI2 and treated with 10 mM CaCl2 for 2 hours, followed by 10 uM Ionomycin for 2 hours
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney tranformed with large T antigen) whole cell lysate transfected with GFP- tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, followed by 10 uM Ionomycin for 2 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 4 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
According to Uniprot annotation, isoform A1-A (34kD) is twenty times more abundant than isoform A1-B (39kD). PADs (including PADI2 and PADI4) are the enzymes that hydrolyze arginine to form citrulline, and this catalytic reaction also needs Ca2+ and ionomycin. Please refer to PMID: 26360112.
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Dot blot analysis of hnRNP A1 (citrulline R97) labeled with ab208028 at 1/1000 dilution.
Lane 1: hnRNP A1 (citrulline R97) peptide.
Lane 2:hnRNP A1 (citrulline R97) non-citrulline peptide.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
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hnRNP A1 (citrulline R97) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate with ab208028 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208028 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate 10 µg (Input).
Lane 2: ab208028 IP in HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208028 in HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate.Exposure time: 10 seconds.
Blocking and dilution buffer: 5% NFDM/TBST.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab208028 は論文での使用が確認できていません。