Anti-hnRNP A1 抗体 (ab4791)
Key features and details
- Rabbit polyclonal to hnRNP A1
- Suitable for: IHC-P, IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-hnRNP A1 antibody
hnRNP A1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to hnRNP A1 -
由来種
Rabbit -
特異性
This antibody recognises hnRNPA1 (39kDa) in Western blots. -
アプリケーション
適用あり: IHC-P, IP, ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Macaque monkey
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Recombinant Human hnRNP A1 (isoform A1-A) protein (ab91691) can be used as a positive control in WB. This antibody gave a positive signal in HeLa whole cell lysate and Human normal skin tissue section. ICC/IF positive control: U2OS cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4791の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).
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| 特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa). |
ターゲット情報
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機能
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. -
配列類似性
Contains 2 RRM (RNA recognition motif) domains. -
翻訳後修飾
Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated. -
細胞内局在
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner. - Information by UniProt
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参照データベース
- Entrez Gene: 3178 Human
- Entrez Gene: 15382 Mouse
- Entrez Gene: 29578 Rat
- Omim: 164017 Human
- SwissProt: P09651 Human
- SwissProt: P49312 Mouse
- SwissProt: P04256 Rat
- Unigene: 546261 Human
see all -
別名
- HNRNPA 1 antibody
- Helix destabilizing protein antibody
- Helix-destabilizing protein antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody (ab4791)
ab4791 staining hnRNP A1 in U2OS cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab4791 at 5µg/ml and ab195883, Rat monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). The secondary antibody (shown in red) was ab150083, Alexa Fluor® 647 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Western blot - Anti-hnRNP A1 antibody (ab4791)All lanes : Anti-hnRNP A1 antibody (ab4791) at 1/500 dilution
Lane 1 : HeLa Whole Cell Extract
Lane 2 : HeLa Nuclear Extract
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?Rabbit polyclonal to hnRNPA1 (ab4791) at 1/500.
Lane 1: HeLa Whole Cell Extract
Lane 2: HeLa Nuclear ExtractSecondary ab: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000)
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Immunoprecipitation - Anti-hnRNP A1 antibody (ab4791)hnRNP A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to hnRNP A1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab4791.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 36kDa: hnRNP A1. -
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody (ab4791)This image is courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UKImmunofluorescent imaging of human cells (U2OS) with ab4791 reveals
the expected ribonucleoprotein particular staining in the nucleus.IF was performed with a standard paraformaldehyde technique (fixed in
PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS
for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/200 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. Nuclei counterstained with Hoechst stain (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody (ab4791)IHC image of hnRNP A1 staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4791, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab4791 は 7 報の論文で使用されています。
- Han X et al. p300-Catalyzed Lysine Crotonylation Promotes the Proliferation, Invasion, and Migration of HeLa Cells via Heterogeneous Nuclear Ribonucleoprotein A1. Anal Cell Pathol (Amst) 2020:5632342 (2020). PubMed: 33457194
- Chen C et al. LNMAT1 promotes lymphatic metastasis of bladder cancer via CCL2 dependent macrophage recruitment. Nat Commun 9:3826 (2018). PubMed: 30237493
- Modelska A et al. The malignant phenotype in breast cancer is driven by eIF4A1-mediated changes in the translational landscape. Cell Death Dis 6:e1603 (2015). WB ; Human . PubMed: 25611378
- Rappe U et al. Nuclear ARVCF protein binds splicing factors and contributes to the regulation of alternative splicing. J Biol Chem 289:12421-34 (2014). Human . PubMed: 24644279
- Hope NR & Murray GI The expression profile of RNA-binding proteins in primary and metastatic colorectal cancer: relationship of heterogeneous nuclear ribonucleoproteins with prognosis. Hum Pathol 42:393-402 (2011). IHC-P ; Human . PubMed: 21194727
- Zhang L et al. Quantitative proteomics analysis reveals BAG3 as a potential target to suppress severe acute respiratory syndrome coronavirus replication. J Virol 84:6050-9 (2010). WB ; Hamster . PubMed: 20392858
- Bowick GC et al. Analysis of the differential host cell nuclear proteome induced by attenuated and virulent hemorrhagic arenavirus infection. J Virol 83:687-700 (2009). WB ; Mouse . PubMed: 19004951
