Anti-HMW Cytokeratin 抗体 [34bE12] (ab776)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)

Key features and details

  • Mouse monoclonal [34bE12] to HMW Cytokeratin
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-HMW Cytokeratin antibody [34bE12]

  • 製品の詳細

    Mouse monoclonal [34bE12] to HMW Cytokeratin

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Solubilized keratin extract from human stratum corneum.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報
    IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
    Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to immunostaining.

    • ターゲット情報

    • 関連性

      Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue. There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).

    • 細胞内局在

      Cytoplasmic

    • 参照データベース

      see all

    • 別名

      • CK13 antibody
      • CK14 antibody
      • CK15 antibody
      • CK16 antibody
      • Cytokeratin 13 antibody
      • Cytokeratin 14 antibody
      • Cytokeratin 15 antibody
      • Cytokeratin 16 antibody
      • K13 antibody
      • K14 antibody
      • K15 antibody
      • K16 antibody
      • Keratin 13 antibody
      • Keratin 14 antibody
      • Keratin 15 antibody
      • Keratin 15, basic antibody
      • Keratin 15, beta antibody
      • Keratin 16 antibody
      • KRT13 antibody
      • KRT14 antibody
      • KRT15 antibody
      • KRT16 antibody
      • KRT16A antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)

      ab776 - immunohistochemistry

      Formalin fixed paraffin embedded human colon carcinoma stained with HMW Cytokeratin, using ABC and AEC chromagen.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)

      ab776 staining human skin tissue sections by IHC-P.

    参考文献 (1)

    ab776 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab776 は 1 報の論文で使用されています。

    • Hagman Z  et al. miR-205 negatively regulates the androgen receptor and is associated with adverse outcome of prostate cancer patients. Br J Cancer 108:1668-76 (2013). IHC ; Human . PubMed: 23571738

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