Anti-HMW Cytokeratin 抗体 [34bE12] (ab776)
製品の概要
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製品名
Anti-HMW Cytokeratin antibody [34bE12] -
製品の詳細
Mouse monoclonal [34bE12] to HMW Cytokeratin -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, IHC-Frmore details -
種交差性
交差種: Human -
免疫原
Solubilized keratin extract from human stratum corneum.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
34bE12 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | ||
| IHC-Fr |
追加情報
IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to immunostaining.
Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to immunostaining.
ターゲット情報
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関連性
Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue. There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46). -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 3860 Human
- Entrez Gene: 3861 Human
- Entrez Gene: 3866 Human
- Entrez Gene: 3868 Human
- Omim: 148030 Human
- Omim: 148065 Human
- Omim: 148066 Human
- Omim: 148067 Human
see all -
別名
- CK13 antibody
- CK14 antibody
- CK15 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)](https://www.abcam.co.jp/ps/products/0/ab776/Images/ab776_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)ab776 - immunohistochemistry
Formalin fixed paraffin embedded human colon carcinoma stained with HMW Cytokeratin, using ABC and AEC chromagen.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)](https://www.abcam.co.jp/ps/products/0/ab776/Images/ab776-3705-anti-hmw-cytokeratin-antibody-34be12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMW Cytokeratin antibody [34bE12] (ab776)ab776 staining human skin tissue sections by IHC-P.
データシートおよび資料
参考文献
This product has been referenced in:
- Hagman Z et al. miR-205 negatively regulates the androgen receptor and is associated with adverse outcome of prostate cancer patients. Br J Cancer 108:1668-76 (2013). IHC ; Human . Read more (PubMed: 23571738) »
