Anti-HMGB2 抗体 (ab67282)
Key features and details
- Rabbit polyclonal to HMGB2
- Suitable for: ICC, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HMGB2 antibody
HMGB2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HMGB2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WB, IPmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Opossum -
免疫原
Synthetic peptide:
KGKSEAGKKGPGRPTGS
conjugated to KLH, corresponding to amino acids 165-181 of Human HMGB2 -
ポジティブ・コントロール
- WB: HEK-293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67282の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC |
Use at an assay dependent concentration.
|
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
|
IP |
Use at an assay dependent concentration. Use 5-10µg.
|
特記事項 |
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ICC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. |
IP
Use at an assay dependent concentration. Use 5-10µg. |
ターゲット情報
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機能
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). -
配列類似性
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 540444 Cow
- Entrez Gene: 3148 Human
- Entrez Gene: 97165 Mouse
- Entrez Gene: 397130 Pig
- Entrez Gene: 29395 Rat
- Entrez Gene: 498072 Rat
- Omim: 163906 Human
- SwissProt: P40673 Cow
see all -
別名
- C80539 antibody
- High mobility group (nonhistone chromosomal) protein 2 antibody
- High mobility group box 2 antibody
see all
画像
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Immunocytochemistry analysis of HUVEC cells using Anti-HMGB2 antibody (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).
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All lanes : Anti-HMGB2 antibody (ab67282) at 2 mg/ml
Lane 1 : NIH3T3
Lane 2 : Rat1
Lane 3 : RINSH
Lane 4 : PC12
Lane 5 : K562
Lane 6 : HEK-293T
Lane 7 : HeLa nuclear
Lane 8 : HeLa
Predicted band size: 24 kDa -
Nuclear localization of HMGB2 in PC12 cells.
A. Cells were stained with Rabbit Anti-HMGB2 (ab67282) at 1 µg/mL
B. DAPI staining
C. Merged staining of HMGB2 and DAPI indicating nuclear staining of HMGB2 -
Lanes
1: 5 µg of Rabbit Anti-HMGB2 (ab67282) were used to immunoprecipitate HMGB2 from HEK-293T cell lysate
2: Negative control – without cell lysate
3: Negative control – without antibody
4: Positive control – Extract of HEK-293T direct labeling
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab67282 は 30 報の論文で使用されています。
- Sato M et al. The lncRNA Caren antagonizes heart failure by inactivating DNA damage response and activating mitochondrial biogenesis. Nat Commun 12:2529 (2021). PubMed: 33953175
- Sofiadis K et al. HMGB1 coordinates SASP-related chromatin folding and RNA homeostasis on the path to senescence. Mol Syst Biol 17:e9760 (2021). PubMed: 34166567
- Wang W et al. LINC00184 plays an oncogenic role in non-small cell lung cancer via regulation of the miR-524-5p/HMGB2 axis. J Cell Mol Med 25:9927-9938 (2021). PubMed: 34651416
- Tan HY et al. Downregulation of miR-223 promotes HMGB2 expression and induces oxidative stress to activate JNK and promote autophagy in an in vitro model of acute lung injury. J Inflamm (Lond) 18:29 (2021). PubMed: 34732212
- Li J et al. Single-cell RNA Sequencing Reveals Thoracolumbar Vertebra Heterogeneity and Rib-genesis in Pigs. Genomics Proteomics Bioinformatics 19:423-436 (2021). PubMed: 34775075