Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Sample
Human Cell (Human prostate cancer cell line (PC-3))
Specification
Human prostate cancer cell line (PC-3)
Permeabilization
Yes - cooled acetone for 1' at -20C
Fixative
Methanol
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in pbs
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5
Dilution
1/300
Additional data
Additional Notes
HMGB1 (yellow), actin (red), DAPI (blue)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 31 2014