Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: PBS plus 1xcasein
Dilution
1/2500
Secondary antibody
Name
Non-Abcam antibody was used: ImmPRESS™ HRP Universal Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Note: The 2nd antibody is ImmPRESS™ HRP Universal Antibody (Anti-Mouse IgG/Anti-Rabbit IgG, Peroxidase) Polymer Detection Kit which reacts with either mouse or rabbit primaries.
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投稿 Jul 16 2019