Key features and details
- Rabbit polyclonal to HMGA1a / HMGA1b - ChIP Grade
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品名Anti-HMGA1a / HMGA1b antibody - ChIP Grade
HMGA1a / HMGA1b 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to HMGA1a / HMGA1b - ChIP Grade
特異性This antibody detects, by Western blot on human GFP fusion proteins in cell lysate, both HMGA1a and the splice variant HMGA1b, but does not detect HMGA2 (see review and Western blot picture). The antibody has not yet been successfully used to detect endogenous protein.
アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
交差が予測される動物種: Mouse, Rat, Chicken, Zebrafish, Neurospora crassa, Chinese hamster
Synthetic peptide corresponding to Human HMGA1a/ HMGA1b aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive result in human placenta FFPE tissue sections
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
精製度Immunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab4078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11.5 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/250 - 1/500.
Although this antibody gives a specific signal in Immunofluoresence, we have had suggestions that strong background signals are seen. See review by Robert Hock and IF picture below.
機能HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
関連疾患Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51L1.
配列類似性Belongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains.
翻訳後修飾Constitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoform HMG-Y can be phosphorylated by HIPK2.
HMG-Y is not methylated.
Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
- Information by UniProt
- High mobility group AT hook 1 antibody
- High mobility group AT-hook protein 1 antibody
- High mobility group protein A1 antibody
Lanes 1-4 : Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078) at 1/1000 dilution
Lanes 5-8 : Anti-GFP
Lane 1 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a GFP fusion proteins.
Lanes 2 & 6 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1b-GFP fusion proteins
Lanes 3 & 7 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA2-GFP fusion proteins
Lane 4 : hole cell lysate from HepG2 cells transfected with plasmids coding for NLS-GFP fusion proteins
Lane 5 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a-GFP fusion proteins
Lane 8 : Whole cell lysate from HepG2 cells transfected with plasmids coding for NLS-GFP fusion proteins
All lanes : Anti-rabbit HRP at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 11.5 kDa
Western blot using ab4078.
Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a-, hHMGA1b-, hHMGA2- or NLS- (nuclear localisation signal) GFP fusion proteins.
ab4078 was diluted 1/1000 in 5% milk/TBS and incubated for 1 hr at room temperature. Secondary HRP anti-rabbit antibody was diluted 1/10000 in 5% milk/TBS and incubated for 1 hr at room temperature.
Lane 1: hHMGA1a-GFP (ab4078)
Lane 2: hHMGA1b-GFP (ab4078)
Lane 3: hHMGA2-GFP (ab4078)
Lane 4: NLS-GFP (ab4078)
Lane 5: hHMGA1a-GFP (anti-GFP)
Lane 6: hHMGA1b-GFP (anti-GFP)
Lane 7: hHMGA2-GFP (anti-GFP)
Lane 8: NLS-GFP (anti-GFP)
The detected size is that expected for the fusion of an 11kD and 26kD protein (GFP).
Immunofluoresence on MCF-7 cells transfected with human HMGA1a-GFP using ab4078.
A: Hoechst stain
C: TexasRed HMG-I/HMG-Y (ab4078)
IHC image of ab4078 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4078, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab4078 は 22 報の論文で使用されています。
- Teng K et al. KIFC1 is activated by TCF-4 and promotes hepatocellular carcinoma pathogenesis by regulating HMGA1 transcriptional activity. J Exp Clin Cancer Res 38:329 (2019). PubMed: 31340839
- Tolza C et al. AP-1 Signaling by Fra-1 Directly Regulates HMGA1 Oncogene Transcription in Triple-Negative Breast Cancers. Mol Cancer Res 17:1999-2014 (2019). PubMed: 31300541
- Patel ZH et al. A plausibly causal functional lupus-associated risk variant in the STAT1-STAT4 locus. Hum Mol Genet 27:2392-2404 (2018). PubMed: 29912393
- Zhang H et al. Mandatory role of HMGA1 in human airway epithelial normal differentiation and post-injury regeneration. Oncotarget 9:14324-14337 (2018). PubMed: 29581847
- Parry AJ et al. NOTCH-mediated non-cell autonomous regulation of chromatin structure during senescence. Nat Commun 9:1840 (2018). PubMed: 29743479