Anti-HLTF 抗体 (ab17984)
Key features and details
- Rabbit polyclonal to HLTF
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HLTF antibody
HLTF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HLTF -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide corresponding to HLTF aa 600-700.
Database link: 6596 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antibody was affinity purified using an epitope specific to SMARCA3 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17984の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/3000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 114 kDa).
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特記事項 |
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WB
1/3000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 114 kDa). |
ターゲット情報
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機能
Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA. -
組織特異性
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 RING-type zinc finger. -
細胞内局在
Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone. - Information by UniProt
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参照データベース
- Entrez Gene: 6596 Human
- Entrez Gene: 20585 Mouse
- Entrez Gene: 295568 Rat
- Omim: 603257 Human
- SwissProt: Q14527 Human
- SwissProt: Q6PCN7 Mouse
- Unigene: 3068 Human
- Unigene: 209650 Mouse
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別名
- DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
- Helicase like transcription factor antibody
- Helicase-like transcription factor antibody
see all
画像
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All lanes : Anti-HLTF antibody (ab17984) at 0.5 µg/ml
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : TCMK-1 whole cell lysate
Lane 3 : 4T1 whole cell lysate
Lane 4 : CT26.WT whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 114 kDa -
Detection of SMARCA3 by western blot and immunoprecipitation.
Lane 1: 20 g of HeLa nuclear extractµ
Lane 2: 50µ g of HeLa nuclear extract
LAne 3: 1 mg of HeLa nuclear extract immunoprecipitated by anti-SMARCA3 (ab17984), used at 4 g/mg of lysate.µ
Anti-SMARCA3 (ab17984) was used for western blot analysis at 1/5,000.
Detection: chemiluminescence with an exposure time of 4 minutes.Detection of SMARCA3 by western blot and immunoprecipitation.
Lane 1: 20µg of HeLa nuclear extract
Lane 2: 50µg of HeLa nuclear extract
LAne 3: 1 mg of HeLa nuclear extract immunoprecipitated by anti-SMARCA3 (ab17984), used at 4µg/mg of lysate.
Anti-SMARCA3 (ab179
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab17984 は 13 報の論文で使用されています。
- Matkovic R et al. TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. Nat Commun 13:66 (2022). PubMed: 35013187
- Kanao R et al. RFWD3 and translesion DNA polymerases contribute to PCNA modification-dependent DNA damage tolerance. Life Sci Alliance 5:N/A (2022). PubMed: 35905994
- Helmer RA et al. Helicase-like transcription factor-deletion from the tumor microenvironment in a cell line-derived xenograft model of colorectal cancer reprogrammed the human transcriptome-S-nitroso-proteome to promote inflammation and redirect metastasis. PLoS One 16:e0251132 (2021). PubMed: 34010296
- Kim JJ et al. PCAF-Mediated Histone Acetylation Promotes Replication Fork Degradation by MRE11 and EXO1 in BRCA-Deficient Cells. Mol Cell 80:327-344.e8 (2020). PubMed: 32966758
- Ostrovsky O et al. Identification of strong intron enhancer in the heparanase gene: effect of functional rs4693608 variant on HPSE enhancer activity in hematological and solid malignancies. Oncogenesis 7:51 (2018). PubMed: 29955035