Anti-HLA G 抗体 [MEM-G/11] (ab4570)

Submit a review Q&A (2)References (3)

Key features and details

  • Mouse monoclonal [MEM-G/11] to HLA G
  • Suitable for: IP, Flow Cyt, ELISA
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-HLA G antibody [MEM-G/11]
    HLA G 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MEM-G/11] to HLA G

  • 由来種

    Mouse

  • 特異性

    HLA G antigen.

  • アプリケーション

    適用あり: IP, Flow Cyt, ELISAmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein (Human) refolded with beta 2 microglobulin and peptide.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    MEM-G/11

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

The antibody MEM-G/11 does not cross-block standard MEM-G/9 antibody.

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.

  • 組織特異性

    Expressed in trophoblasts.

  • 配列類似性

    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B2 microglobulin antibody
      • DADB-15K14.8 antibody
      • HLA 6.0 antibody
      • HLA class I histocompatibility antigen alpha chain G antibody
      • HLA class I histocompatibility antigen, alpha chain G antibody
      • HLA class I molecule antibody
      • HLA G antibody
      • HLA G antigen antibody
      • HLA G histocompatibility antigen class I G antibody
      • HLA G3 antibody
      • HLA-G antibody
      • HLA-G histocompatibility antigen, class I antibody
      • HLA60 antibody
      • HLAG antibody
      • HLAG_HUMAN antibody
      • Major histocompatibility complex class I G antibody
      • MHC class I antigen antibody
      • MHC class I antigen G antibody
      • MHC G antibody
      • T-cell A locus antibody
      • TCA antibody
      see all

    参考文献 (3)

    ab4570 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4570 は 3 報の論文で使用されています。

    • Apps R  et al. A homodimeric complex of HLA-G on normal trophoblast cells modulates antigen-presenting cells via LILRB1. Eur J Immunol 37:1924-37 (2007). WB . PubMed: 17549736
    • Menier C  et al. Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol 64:315-26 (2003). PubMed: 12590976
    • Boyson JE  et al. Disulfide bond-mediated dimerization of HLA-G on the cell surface. Proc Natl Acad Sci U S A 99:16180-5 (2002). PubMed: 12454284

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    One of our customers wishes to simultaneously bind 2 antibodies to magnetic beads for IP. I do not see why there should be any problem with this procedure.
    Do you foresee any cross reactivity or specicific problems with the binding both these antibodies to magnetic beads?
    ab42454, ab4570.

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    Answer




    I don't expect any problems with the ab4570 as this one works fine in IP as well, however, I am not sure about the ab42454.

    Nonetheless, I am not too concerned either: ab42454 is not tested in IP, but it works in ELISA, which is a good indicator that it will react with the native protein.

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    Question

    Does this antibody recognize any of the isotypes (G1, G2, G3, G4)? For instance, clone MEM-G9 specificities was demonstrated with cells transfected with either full-length HLA-G and HLA-G1 complementary DNA.

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    Answer

    Antibodies MEM-G/9 and MEM-G/11 recognize HLA-G1 (full length) but not truncated isoforms (G2, G3, G4) on the cell surface. Both react with HLA-G5 (G6 was not rigorously tested), i.e. the soluble isoform. MEM-G/11 reacts with a different epitope than MEM-G/9, this could be useful in double staining (MEM-G/11 is available as FITC and MEM-G/9 as PE conjugate) of cell surface HLA-G1. In a soluble form (actively secreted G5 or shedded G1), it was not demonstrated that both mAbs can be used as pair in sandwich ELISA.

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