Anti-HLA E 抗体 [MEM-E/07] (ab11820)
Key features and details
- Mouse monoclonal [MEM-E/07] to HLA E
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-HLA E antibody [MEM-E/07]
HLA E 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MEM-E/07] to HLA E -
由来種
Mouse -
特異性
The antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules: HLA B7 (strongly), HLA B8 (moderately), HLA B27, HLA-B44 (weakly). -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to HLA E. The epitope is extracellular. The antibody reacts only with native epitope, not denatured.
Database link: P13747 -
ポジティブ･コントロール
- IHC-P: Human spleen tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from hybridoma culture supernatant by protein A-affinity chromatography. -
ポリ/モノ
モノクローナル -
クローン名
MEM-E/07 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11820の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
|
IHC-P
Use a concentration of 10 µg/ml. |
ターゲット情報
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関連性
HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. -
細胞内局在
Membrane; Single-pass type I membrane protein -
参照データベース
- Entrez Gene: 3133 Human
- Omim: 143010 Human
- SwissProt: P13747 Human
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別名
- HLA class I histocompatibility antigen E alpha chain antibody
- EA1.2 antibody
- EA2.1 antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab11820 は 7 報の論文で使用されています。
- Sasaki T et al. Microenvironmental stresses induce HLA-E/Qa-1 surface expression and thereby reduce CD8(+) T-cell recognition of stressed cells. Eur J Immunol 46:929-40 (2016). PubMed: 26711740
- Shwetank et al. Inhibition of ERK and proliferation in NK cell lines by soluble HLA-E released from Japanese encephalitis virus infected cells. Immunol Lett 162:94-100 (2014). PubMed: 25086398
- Shwetank et al. Infection of human endothelial cells by Japanese encephalitis virus: increased expression and release of soluble HLA-E. PLoS One 8:e79197 (2013). Human . PubMed: 24236107
- Jagessar SA et al. Unravelling the T-cell-mediated autoimmune attack on CNS myelin in a new primate EAE model induced with MOG34-56 peptide in incomplete adjuvant. Eur J Immunol 42:217-27 (2012). PubMed: 21928277
- Jørgensen PB et al. Epstein-Barr virus Peptide Presented by HLA-E is Predominantly Recognized by CD8(bright) Cells in multiple Sclerosis Patients. PLoS One 7:e46120 (2012). Flow Cyt . PubMed: 23049954
- Park B et al. The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradation. J Exp Med 207:2033-41 (2010). WB ; Human . PubMed: 20713594
- Palmisano GL et al. HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol 66:1-12 (2005). PubMed: 15620456