Anti-HLA-DPB1 抗体 [EPR11226] (ab157210)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11226] to HLA-DPB1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HLA-DPB1 antibody [EPR11226]
HLA-DPB1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11226] to HLA-DPB1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IPmore details
適用なし: Flow Cyt -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human fetal thymus and Human tonsil lysates; Human tonsil tissue; Jurkat cells; Immunoprecipitation pellet from fetal thymus lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11226 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157210の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 29 kDa.
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IHC-P | (1) |
1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified, use 1/100 - 1/250. |
ICC/IF |
1/50 - 1/250.
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IP |
1/10 - 1/100.
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特記事項 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 29 kDa. |
IHC-P
1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified, use 1/100 - 1/250. |
ICC/IF
1/50 - 1/250. |
IP
1/10 - 1/100. |
ターゲット情報
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機能
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. -
配列類似性
Belongs to the MHC class II family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation. - Information by UniProt
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参照データベース
- Entrez Gene: 3115 Human
- SwissProt: P04440 Human
- Unigene: 485130 Human
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別名
- beta1 domain MHC class II HLA DPB antibody
- class II histocompatibility antigen, DP(W4) beta chain antibody
- class II HLA beta chain antibody
see all
画像
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Immunohistochemical staining of paraffin embedded human tonsil with purified ab157210 at a working dilution of 1/2500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunofluorescent analysis of Jurkat cells labeling MHC Class II with unpurified ab157210 at 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MHC Class II with unpurified ab157210 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-HLA-DPB1 antibody [EPR11226] (ab157210) at 1/20000 dilution (purified)
Lane 1 : Human fetal thymus lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human fetal spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
ab157210 (purified) at 1/70 immunoprecipitating MHC Class II in 10 μg Daudi cell lysate (Lanes 1 and 2, observed at 29 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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Immunofluorescence staining of Raji cells with purified ab157210 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab157210 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab157210 at a working dilution of 1/2500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Unpurified ab157210 showing positiveve staining in human normal colon.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Unpurified ab157210 showing negative staining in Human heart.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Unpurified ab157210 showing negative staining in Human normal brain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-HLA-DPB1 antibody [EPR11226] (ab157210) at 1/10000 dilution (Unpurified)
Lane 1 : Human fetal thymus cell lysate
Lane 2 : Human tonsil cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa -
Anti-HLA-DPB1 antibody [EPR11226] (ab157210) at 1/10000 dilution (Unpurified) + Immunoprecipitation pellet from Human fetal thymus lysate at 10 µg
Predicted band size: 29 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab157210 は 16 報の論文で使用されています。
- Godfrey J et al. Pembrolizumab for the treatment of disease relapse after allogeneic hematopoietic stem cell transplantation. Blood Adv 7:963-970 (2023). PubMed: 35973200
- Bradley D et al. Interferon gamma mediates the reduction of adipose tissue regulatory T cells in human obesity. Nat Commun 13:5606 (2022). PubMed: 36153324
- Jeong HO et al. Cellular plasticity and immune microenvironment of malignant pleural effusion are associated with EGFR-TKI resistance in non-small-cell lung carcinoma. iScience 25:105358 (2022). PubMed: 36339256
- Johnson BE et al. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer. Cell Rep Med 3:100525 (2022). PubMed: 35243422
- Clotet-Freixas S et al. Extracellular Matrix Injury of Kidney Allografts in Antibody-Mediated Rejection: A Proteomics Study. J Am Soc Nephrol 31:2705-2724 (2020). PubMed: 32900843