Anti-HLA-DP 抗体 [B7/21] (ab20897)

Thank you very much for your reply.

The binding affinity and mechanism varies between blocking antibodies, butthey do seem to generally act as antagonists. These kinds of characteristics need to be determined for each individual antibody and antigen. For the HLA antibody Ab23632, we only have one publication demonstrating its use in a blocking experiment and there doesn't seem to be any data regarding the binding affinity of the antibody-

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1069546/

The specific time the antibody will bind to a single antigen depends on factors of the experiment such as temperature, concentration of the antibody in the media, cell type, etc. I wouldn't expect the blocking to be effective for many days, but probably the effect will be noticeable on the hour-scale.

I am sorry that we don't havemore information regarding this, but if you have further questions or need anything else, please let me know and I'll be happy to help.

Thank you again for contacting us and for your patience while I've been in touch with the lab about your enquiry.

Ab20897 against HLA-DP has not been tested in a blocking experiment at this time, though it is possible that it will work in such an assay. Unfortunately none of our other HLA-DP antibodies seem tohave been tested for blocking ability atthis time. If you're interested in trying Ab20897 in a blocking experiment, we do have a testing discount programand I'd be happy to send more information about this if you're interested.

The clone SPV-L3 against HLA-DQ, which we carry as Ab23632, has been used in a blocking experiment in the following publication-

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1069546/

We would recommend removing the sodium azide from Ab23632 before incubating it with live cells, as azide will cause cell death. An antibody purification kit such as Ab102784 can be used to remove the azide-

https://www.abcam.com/Antibody-Purification-Kit-Protein-A-ab102784.html

I hope that this information is useful, but please let me know if you have any further questions and I'll be happy to help.

Thank you very much for contacting us with your enquiry.

Theterm "functional studies" can be used to describe several different kinds of assays, and sometimes blocking assays are included in this categorization so there isn't a specific difference between blocking and functional studies antibodies.

For example, ab136320 is listed as being tested in functional studies and a blocking assay, however after checking through our internal notes I see that the functional study was actually a blocking assay. Ab23755 was also tested in a functional study, however this was a tissue typing and crossmatching experiment,not a blocking assay. The details of the functional study are often on the datasheet in the "Application Notes" section, however this is not always the case. I apologize that these designationsare not more straight-forward and clear.

Are you using human samples? Ab136320 will be a good antibody for blocking HLA DR, but we don't currently have any antibodies listed as being tested for blocking of HLA DP and HLA DQ. There are two antibodies to these targets that might work in such an assay, ab20897 (HLA DP) and ab23632 (HLA DQ), but I'm checking with the lab to make sure they've been tested for blocking ability. I'll get back to you as soon as I have confirmed this.

I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help.