Key features and details
- Mouse monoclonal [MEM-147] to HLA Class I
- Suitable for: Flow Cyt, IP, WB
- Reacts with: Human
- Isotype: IgG1
製品名Anti-HLA Class I antibody [MEM-147]
HLA Class I 一次抗体 製品一覧
製品の詳細Mouse monoclonal [MEM-147] to HLA Class I
特異性Reacts with all human classical MHC Class I molecules in native cell-surface forms. Positive in immunoblotting with many (not all) MHC Class I allelic forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). Positive in Western blotting (non-reducing conditions) with most HLA-B and HLA-C molecules, but not HLA-A. Epitope specificity is very similar to classical antibody W6/32 (ab7855).
アプリケーション適用あり: Flow Cyt, IP, WBmore details
PHA-activated peripheral blood lymphocytes
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.097% Sodium azide
Concentration information loading...
精製度Protein A purified
特記事項（精製）Purified from TCS. Purity >95% by SDS-PAGE.
Our Abpromise guarantee covers the use of ab9090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|WB||1/100. Use non-reducing conditions.|
関連性HLA CLass I is involved in the presentation of foreign antigens to the immune system.
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
Anti-HLA Class I antibody [MEM-147] (ab9090) at 1/100 dilution + Human Ramos B cell line lysates (1% laurylmaltoside)
Goat anti-mouse IgG (H+L)-HRP conjugate
Performed under non-reducing conditions.
Overlay histogram showing Jurkat cells stained with ab9090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9090, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Jurkat cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
ab9090 は 1 報の論文で使用されています。
- Tran TM et al. The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics 53:440-6 (2001). PubMed: 11685454