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  1. Link

    hla-class-i-antibody-mem-147-ab9090.pdf

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Immunology Adaptive Immunity MHC Class I
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Anti-HLA Class I 抗体 [MEM-147] (ab9090)

  • Datasheet
  • SDS
Submit a review Q&A (5)References (1)

Product price, shipping and contact information

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-HLA Class I antibody [MEM-147] (ab9090)
  • Flow Cytometry - Anti-HLA Class I antibody [MEM-147] (ab9090)

Key features and details

  • Mouse monoclonal [MEM-147] to HLA Class I
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

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HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-HLA Class I antibody [MEM-147]
    HLA Class I 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [MEM-147] to HLA Class I
  • 由来種

    Mouse
  • 特異性

    Reacts with all human classical MHC Class I molecules in native cell-surface forms. Positive in immunoblotting with many (not all) MHC Class I allelic forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). Positive in Western blotting (non-reducing conditions) with most HLA-B and HLA-C molecules, but not HLA-A. Epitope specificity is very similar to classical antibody W6/32 (ab7855).
  • アプリケーション

    適用あり: Flow Cyt, WBmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Tissue, cells or virus corresponding to HLA Class I. PHA-activated peripheral blood lymphocytes

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • 特記事項(精製)

    Purified from TCS. Purity >95% by SDS-PAGE.
  • ポリ/モノ

    モノクローナル
  • クローン名

    MEM-147
  • アイソタイプ

    IgG1
  • 研究分野

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class I

関連製品

  • Alternative Versions

    • PE Anti-HLA Class I antibody [MEM-147] (ab52451)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9090の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
1/100. Use non-reducing conditions.
特記事項
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
1/100. Use non-reducing conditions.

ターゲット情報

  • 関連性

    HLA CLass I is involved in the presentation of foreign antigens to the immune system.
  • 細胞内局在

    Plasma membrane
  • 参照データベース

    • Entrez Gene: 3105 Human
    • Entrez Gene: 3133 Human
    • Entrez Gene: 3134 Human
    • Entrez Gene: 3135 Human
    • Omim: 142800 Human
    • SwissProt: O15506 Human
    • SwissProt: P04439 Human
    • SwissProt: Q5MCQ6 Human
    • SwissProt: Q5ZGM8 Human
    • SwissProt: Q7YQ33 Human
    • SwissProt: Q861B7 Human
    • SwissProt: Q8MHP7 Human
    • SwissProt: Q9MY64 Human
    • SwissProt: Q9MY72 Human
    • SwissProt: Q9MYB7 Human
    see all
  • 別名

    • DKFZp686P19218 antibody
    • EA1.2 antibody
    • EA2.1 antibody
    • HLA 5.4 antibody
    • HLA 6.2 antibody
    • HLA A antibody
    • HLA B antibody
    • HLA C antibody
    • HLA CDA12 antibody
    • HLA F antibody
    • HLA G antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLAF antibody
    • Major histocompatibility complex class I A antibody
    • Major histocompatibility complex class I antibody
    • Major histocompatibility complex class I E antibody
    • Major histocompatibility complex class I F antibody
    • MHC antibody
    • MHC Class I antigen antibody
    • MHC G antibody
    see all

画像

  • Western blot - Anti-HLA Class I antibody [MEM-147] (ab9090)
    Western blot - Anti-HLA Class I antibody [MEM-147] (ab9090)
    Anti-HLA Class I antibody [MEM-147] (ab9090) at 1/100 dilution + Human Ramos B cell line lysates (1% laurylmaltoside)

    Secondary
    Goat anti-mouse IgG (H+L)-HRP conjugate

    Performed under non-reducing conditions.
  • Flow Cytometry - Anti-HLA Class I antibody [MEM-147] (ab9090)
    Flow Cytometry - Anti-HLA Class I antibody [MEM-147] (ab9090)
    Overlay histogram showing Jurkat cells stained with ab9090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9090, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Jurkat cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

プロトコール

  • Western blot protocols
  • Flow cytometry protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (1)

ab9090 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9090 は 1 報の論文で使用されています。

  • Tran TM  et al. The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics 53:440-6 (2001). PubMed: 11685454

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-5 of 5 Abreviews or Q&A

Question

I am looking for an antibody for FACS analysis that recognizes HLA-A, B or C but not the B2 microglobulin portion of the HLA Class I antigen. I need to know if the actual HLA-A, B or C molecule is expressed on the outside of the cell and if the anitbody recognizes the B2 microglobulin part of the cell, I can't tell if just the B2 microglobulin is expressed without the HLA-A, B or C molecule or not. I am working with stem cells and the current FACS antibody I'm using recognizes the B2 microglobulin portion as well as A, B and C. So even if the cell is only expressing the B2 microglobulin, there would be a very strong postive result, but the cell does not actually express functional HLA-A, B or C. Thank you for your assistance in this matter.

Read More

Abcam community

Verified customer

Asked on Jun 03 2005

Answer

Thank you for your enquiry. For this particular antibody, we unfortunately do not have this information. Our source for ab9090 has also said that they think that a pan MHC antibody that recognizes only the free heavy chains on the surface (where it is often complexed with b2m) is not commercially available anywhere. If you have any additional questions, please contact us again.

Read More

Abcam Scientific Support

Answered on Jun 14 2005

Question

I am looking for an anti HLA classI monoclonal antibody of the IgM isotype. Please, could you let me know if there is one IgM isotype among yours ab 2217 (MEM 123), ab 9090 (MEM 147) or ab 3975 (MEM 81).

Read More

Abcam community

Verified customer

Asked on Feb 18 2005

Answer

Thank you for your email. Ab2217 is isotype IgG3; ab9090 is isotype IgG1; and ab3975 is isotype IgG1. You may also find the isotype information on our online datasheets. If you have any additional questions, please contact us again.

Read More

Abcam Scientific Support

Answered on Feb 18 2005

Question

Customer is interested in using this antibody (ab9090) in IP and WB, and would like to know if it cross-reacts with HLA-G or whether it blocks any other HLA G antibodies. Any information would be greatly appreciated. Thanks!

Read More

Abcam community

Verified customer

Asked on Nov 24 2003

Answer

The supplier gave me the following information regarding your question. If you have any additional questions, please contact us again. The antibody MEM-147 (ab9090) does not react with HLA-G1 overexpressed in transfectants. It can be used for IP experiments-it recognizes most HLA-A,B,C in native conformation. In western blots-NON REDUCED NON BOILED samples only, but no HLA-A will be detected. For details see: Tran T.M. et al., Immunogenetics 53, 440 (2001) - the comparative study with W6/32 antibody.

Read More

Abcam Scientific Support

Answered on Nov 24 2003

Question

I'm interested in a marker for human cells - identifying cells implanted into rodent brains viewed immunohistochemically. The human cells implanted may take on any phenotype so we are not able to rely simply on something like a human specific anti-nerve specific enolase - we could get human cardiac tissue, connective tissue, blood cells, anything! What is the best choice - or range of choices for anti-human markers in such a situation? Thanks

Read More

Abcam community

Verified customer

Asked on Apr 11 2002

Answer

Interesting question, but I'm afraid that we are unable to offer any suggestions. Please let us know if you find a suitable target.

Read More

Abcam Scientific Support

Answered on Apr 15 2002

Question

Hi Abcamteam ! Is this antibody useful for blocking experiments in an MLTC ? Best regards from Mainz Kai Kronfeld

Read More

Abcam community

Verified customer

Asked on Apr 10 2002

Answer

As far as we are aware, this antibody has not yet been tested in this application.

Read More

Abcam Scientific Support

Answered on Apr 10 2002

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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