Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Pulmonary artery conduit)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6 Citrate buffer
Permeabilization
No
Specification
Pulmonary artery conduit
Blocking step
Dako Serum free protein block (casein X0909) as blocking agent for 30 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Dako Antibody Diluent (S0809)
Concentration
4 µg/ml
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
The Dako EnVision+ Dual Link System-HRP DAB kit (K4065) was used for visualization. This antibody did not stain any cells in ovine main pulmonary artery conduit. Similar results were obtained when omitting antigen retrieval and using heat mediated retrieval with a pH 9 solution (Dako S2368).
Abcam response
Thank you so much for testing this antibody in sheep and IHC-P. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species and application.
The customer received a discount on a future antibody purchase for testing this product. We welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 07 2017