Anti-HLA-C 抗体 [EPR6749] - BSA and Azide free (ab240027)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6749] to HLA-C - BSA and Azide free
- Suitable for: Dot blot, IP, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HLA-C antibody [EPR6749] - BSA and Azide free
HLA-C 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6749] to HLA-C - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Dot blot, IP, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Jurkat, HEK-293, A375, and HeLa whole cell lysates. Human lung and lymphoma tissue lysates; IP: Jurkat whole cell lysate. Dot blot: HLA-C peptides
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特記事項
ab240027 is the carrier-free version of ab126722.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6749 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab240027の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Dot blot |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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特記事項 |
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Dot blot
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 3107 Human
- Omim: 142840 Human
- SwissProt: P04222 Human
- SwissProt: P10321 Human
- SwissProt: Q07000 Human
- Unigene: 656020 Human
- Unigene: 743218 Human
- Unigene: 77961 Human
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別名
- 1C07_HUMAN antibody
- Cw-7 alpha chain antibody
- D6S204 antibody
see all
画像
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126722).
Dot blot analysis of HLA-C using ab126722 at 1:1000 (2.193 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.
Lane 1: HLA-A peptide
Lane 2: HLA-B peptide
Lane 3: HLA-C peptide
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds.
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All lanes : Anti-HLA-C antibody [EPR6749] (ab126722) at 1/5000 dilution (Purified)
Lane 1 : Jurkat (Human Tcellleukemia Tlymphocyte) whole cell lysates
Lane 2 : HEK-293 (Human embryonickidney epithelial cell) whole cell lysates
Lane 3 : A375 (Human malignant melanoma epithelial cell) whole cell lysates
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 5 : Human lung lysates
Lane 6 : Human lymphoma lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126722).
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Purified ab126722 at 1/100 dilution (2ug) immunoprecipitating HLA-C in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate (10µg)
Lane 2 (+): ab126722 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126722 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 41 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126722).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab240027 は論文での使用が確認できていません。