Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-HLA B7 抗体 [BB7.1] (ab20169)

Submit a review Q&A (2)References (1)
Flow Cytometry - Anti-HLA B7 antibody [BB7.1] (ab20169)

    Key features and details

    • Mouse monoclonal [BB7.1] to HLA B7
    • Suitable for: Flow Cyt
    • Reacts with: Human, Monkey
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    FITC PE

    製品の概要

    • 製品名

      Anti-HLA B7 antibody [BB7.1]
      HLA B7 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [BB7.1] to HLA B7

    • 由来種

      Mouse

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Human, Monkey

    • 免疫原

      Full length protein corresponding to Human HLA B7. Papain solubilised HLA-A2, B7

    • 特記事項


      Human Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). This antibody can be used for HLA typing.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Constituent: PBS
    • Concentration information loading...

    • 精製度

      Protein G purified

    • 一次抗体 備考

      Human Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). This antibody can be used for HLA typing.

    • ポリ/モノ

      モノクローナル

    • クローン名

      BB7.1

    • ミエローマ

      NS1

    • アイソタイプ

      IgG1

    • 軽鎖の種類

      unknown

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab20169の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    1/10.

    Neat - 1/10.

     

     

     

     

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     
    特記事項
    Flow Cyt
    1/10.

    Neat - 1/10.

     

     

     

     

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    ターゲット情報

    • 関連性

      HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa. Hundreds of HLA B alleles have been described.

    • 細胞内局在

      Cell Membrane, single pass type I membrane protein.

    • 参照データベース

      • 別名

        • Abacavir hypersensitivity, susceptibility to antibody
        • Drug-induced liver injury due to flucloxacillin antibody
        • HLA B antibody
        • HLA class I histocompatibility antigen B 7 alpha chain antibody
        • HLA class I histocompatibility antigen, B-13 alpha chain antibody
        • HLA class I histocompatibility antigen, B-14 alpha chain antibody
        • HLA class I histocompatibility antigen, B-15 alpha chain antibody
        • HLA class I histocompatibility antigen, B-18 alpha chain antibody
        • HLA class I histocompatibility antigen, B-27 alpha chain antibody
        • HLA class I histocompatibility antigen, B-35 alpha chain antibody
        • HLA class I histocompatibility antigen, B-37 alpha chain antibody
        • HLA class I histocompatibility antigen, B-38 alpha chain antibody
        • HLA class I histocompatibility antigen, B-40 alpha chain antibody
        • HLA class I histocompatibility antigen, B-41 alpha chain antibody
        • HLA class I histocompatibility antigen, B-42 alpha chain antibody
        • HLA class I histocompatibility antigen, B-44 alpha chain antibody
        • HLA class I histocompatibility antigen, B-45 alpha chain antibody
        • HLA class I histocompatibility antigen, B-46 alpha chain antibody
        • HLA class I histocompatibility antigen, B-47 alpha chain antibody
        • HLA class I histocompatibility antigen, B-48 alpha chain antibody
        • HLA class I histocompatibility antigen, B-49 alpha chain antibody
        • HLA class I histocompatibility antigen, B-50 alpha chain antibody
        • HLA class I histocompatibility antigen, B-51 alpha chain antibody
        • HLA class I histocompatibility antigen, B-52 alpha chain antibody
        • HLA class I histocompatibility antigen, B-53 alpha chain antibody
        • HLA class I histocompatibility antigen, B-54 alpha chain antibody
        • HLA class I histocompatibility antigen, B-55 alpha chain antibody
        • HLA class I histocompatibility antigen, B-56 alpha chain antibody
        • HLA class I histocompatibility antigen, B-57 alpha chain antibody
        • HLA class I histocompatibility antigen, B-58 alpha chain antibody
        • HLA class I histocompatibility antigen, B-59 alpha chain antibody
        • HLA class I histocompatibility antigen, B-67 alpha chain antibody
        • HLA class I histocompatibility antigen, B-7 alpha chain antibody
        • HLA class I histocompatibility antigen, B-73 alpha chain antibody
        • HLA class I histocompatibility antigen, B-78 alpha chain antibody
        • HLA class I histocompatibility antigen, B-8 alpha chain antibody
        • HLA class I histocompatibility antigen, B-81 alpha chain antibody
        • HLA class I histocompatibility antigen, B-82 alpha chain antibody
        • HLAB antibody
        • Leukocyte antigen class I B antibody
        • Lymphocyte antigen antibody
        • Major histocompatibility complex class I B antibody
        • MHC class I antigen B 7 antibody
        • Synovitis, chronic, susceptibility to antibody
        see all

      画像

      • Flow Cytometry - Anti-HLA B7 antibody [BB7.1] (ab20169)
        Flow Cytometry - Anti-HLA B7 antibody [BB7.1] (ab20169)
        Overlay histogram showing Jurkat cells stained with ab20169 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (a20169, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      参考文献 (1)

      ab20169 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab20169 は 1 報の論文で使用されています。

      • Brodsky FM  et al. Monoclonal antibodies for analysis of the HLA system. Immunol Rev 47:3-61 (1979). PubMed: 95015

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-2 of 2 Abreviews or Q&A

      Question

      Thank you for the information . I would like to know the molecular weights of the antibodies itself.   

      Read More
      Answer

      The exact molecular weight of these antibodies is not known, however, most mammalian antibodies have a total molecular weight of approximately 150kDa. This is broken down into two heavy chains of approximately 50kDa each and to light chains of approximately 25kDa each.

      Read More

      Question

      Hello,    Can I know what are the molecular weights of two of your antibodies: Anti-HLA Class I antibody [W6/32.] (ab7855) Anti-HLA B7 antibody [BB7.1] (ab20169) Thank you, 

      Read More
      Answer

      Thank you for contacting Abcam. According to the information on the website, both ab20169 and ab7855 should recognize a protein that has a molecular weight of approximately 45kDa. If there is anything else I can help you with, please le me know.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com