Application
Western blot
Sample
Human Cell lysate - whole cell (10 ul Supernatant from PBMC culture containing vir)
Loading amount
10 cells
Specification
10 ul Supernatant from PBMC culture containing vir
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T plus 5% nonfat dry milk
Secondary antibody
Name
Non-Abcam antibody was used: Borrowed anti-goat IGg
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 120, 100 kDa
Negative control
Sup from untransfected cells
Additional data
Additional Notes
PVDF membrane
Legend:
1)HIV1 replication competent strain A
2)HIV1 non-replication competent strain A
3)HIV1 replication competent strain B
4)HIV1 replication competent strain C
5)Untransfected
6)Blank (no cells)
Legend:
1)HIV1 replication competent strain A
2)HIV1 non-replication competent strain A
3)HIV1 replication competent strain B
4)HIV1 replication competent strain C
5)Untransfected
6)Blank (no cells)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Hoku West-Foyle
Verified customer
投稿 Nov 06 2009