Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (Whole cell lysate)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
60000 cells
Specification
Whole cell lysate
Blocking step
Odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey blocking buffer
Dilution
1/1000
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: 680RD anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680
Detection
Detection method
IR
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 16 kDa Non-specific: 19 kDa
Positive control
Control cells were considered positive control.
Additional data
Abcam response
Discount code: ABTRIAL-TVSIF
MW 03/8/2016: tested WB, untested S. cerevisiae, ready to publish
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 03 2016