Anti-Histone H4 (tri methyl K20) 抗体 - ChIP Grade (ab9053)
Key features and details
- Rabbit polyclonal to Histone H4 (tri methyl K20) - ChIP Grade
- Suitable for: WB, ChIP
- Reacts with: Mouse, Rat, Cow, Human, Schizosaccharomyces pombe, Toxoplasma gondii
- Isotype: IgG
製品の概要
-
製品名
Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade
Histone H4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Histone H4 (tri methyl K20) - ChIP Grade -
由来種
Rabbit -
特異性
Histone H4 tri-methylated at Lys 20. -
アプリケーション
適用あり: WB, ChIPmore details -
種交差性
交差種: Mouse, Rat, Cow, Human, Schizosaccharomyces pombe, Toxoplasma gondii
交差が予測される動物種: Mammals, Xenopus tropicalis
-
免疫原
Synthetic peptide within Human Histone H4 aa 1-100 (tri methyl K20) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab17567) -
特記事項
In immunofluorescence: Human cells: The antibody stains a subset of cellular chromatin that is characteristically DAPI-rich (condensed, heterochromatic). The perinucleolar heterochromatin is particularly rich in trimethylated lysine 20 staining. Mouse: Prominent staining of a subset of centromeric or pericentromeric heterochromatin. Interphase: The cells within the culture show a considerable variability in the intensity of staining with the antibody. The relationship between trimethylation levels and cell cycle have not yet been determined but may be a contributor to the amount of methylation detected in each cell. Heterochromatic regions of the interphase nucleus are the primary sites of trimethylation observed by indirect immunofluroescence. Mitosis: Discrete chromosomal regions are labelled intensely, with lower level fluorescence throughout the remainder of the chromosome arms. Immunofluorescence staining was performed as part of the nuclear antibody characterisation program at www.cellnucleus.com. Trimenthylation at Lys20 of Histone H4 isa common hallmark of human cancer (Fraga et al. 2005).
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified using Sulpholink column with specific peptide linked via its cysteine residue -
一次抗体 備考
In immunofluorescence: Human cells: The antibody stains a subset of cellular chromatin that is characteristically DAPI-rich (condensed, heterochromatic). The perinucleolar heterochromatin is particularly rich in trimethylated lysine 20 staining. Mouse: Prominent staining of a subset of centromeric or pericentromeric heterochromatin. Interphase: The cells within the culture show a considerable variability in the intensity of staining with the antibody. The relationship between trimethylation levels and cell cycle have not yet been determined but may be a contributor to the amount of methylation detected in each cell. Heterochromatic regions of the interphase nucleus are the primary sites of trimethylation observed by indirect immunofluroescence. Mitosis: Discrete chromosomal regions are labelled intensely, with lower level fluorescence throughout the remainder of the chromosome arms. Immunofluorescence staining was performed as part of the nuclear antibody characterisation program at www.cellnucleus.com. Trimenthylation at Lys20 of Histone H4 isa common hallmark of human cancer (Fraga et al. 2005). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
ChIP Related Products
-
Compatible Secondaries
-
Control Peptide
- Human Histone H3 (mono methyl K4) peptide (ab1340)
- Human Histone H3 (tri methyl K4) peptide (ab1342)
- Human Histone H4 (di methyl K20) peptide (ab14964)
- Human Histone H4 (mono methyl K20) peptide (ab17043)
- Human Histone H3 (mono methyl K9) peptide (ab1771)
- Human Histone H3 (di methyl K9) peptide (ab1772)
- Human Histone H3 (tri methyl K9) peptide (ab1773)
- Human Histone H3 (di methyl K4) peptide (ab7768)
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab9053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa).Can be blocked with Human Histone H4 (tri methyl K20) peptide (ab17567). | |
| ChIP | Use 4-5µg for 106 cells. |
ターゲット情報
-
機能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
配列類似性
Belongs to the histone H4 family. -
翻訳後修飾
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
-
参照データベース
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
別名
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
画像
-
ChIP - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 6 µl of ab9053 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
-
Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)ab9053 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 5 where the activity of ab9053 is specifically blocked by the addition of the immunizing peptide (ab17567).
-
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)This image is courtesy of Michael Hendzel, Cross Cancer Inst, Edmonton, CanadaA mouse 10T1/2 embryonic fibroblast immunofluorescently stained with anti Tri Methyl Lysine 20 of histone H4. Murine 10T1/2 embryonic fibroblasts were cultured on glass coverslips, fixed with paraformaldehyde and then immunofluorescently labeled using an anti-Tri Methyl K20 of histone H4 antibody at 1/500 (green). The nuclear chromatin was simultaneously visualized using DAPI (red).
This image is part of the nuclear antibody characterisation program at www.cellnucleus.com
The image was collected using a 1.4 N.A. 100 X PlanApo Objective (Zeiss) using a Zeiss Axiovert 200M inverted fluorescent microscope and a Photometrics Cascade 16-bit CCD. The images were collected using Universal Imaging MetaMorph v. 5.1 for Windows. A Physik Instruments piezo z-drive was used to collect images at 200 nm intervals throughout the depth of the cell nucleus. The digital images were processed to remove fluorescent background and colour com
-
Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)Left panel: a fission yeast whole cell extract and recombinant (r) fission yeast histone H4.
Right panel: fission yeast whole cell extracts from strains with only a single wt (h4) or mutant (h4K20R) gene were immunoblotted.
Review by Steven Sanders submitted 13 August 2004
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (156)
ab9053 は 156 報の論文で使用されています。
- Liu JY et al. Protective effect of down-regulated microRNA-27a mediating high thoracic epidural block on myocardial ischemia-reperfusion injury in mice through regulating ABCA1 and NF-?B signaling pathway. Biomed Pharmacother 112:108606 (2019). PubMed: 30802823
- Li H et al. Negative Regulation of p53-Induced Senescence by N-WASP Is Crucial for DMBA/TPA-Induced Skin Tumor Formation. Cancer Res 79:2167-2181 (2019). PubMed: 30894371
- Guo X et al. HDAC6 is associated with the formation of aortic dissection in human. Mol Med 25:10 (2019). PubMed: 30925865
- Bikkul MU et al. Telomere elongation through hTERT immortalization leads to chromosome repositioning in control cells and genomic instability in Hutchinson-Gilford progeria syndrome fibroblasts, expressing a novel SUN1 isoform. Genes Chromosomes Cancer 58:341-356 (2019). PubMed: 30474255
- Bröhm A et al. Somatic Cancer Mutations in the SUV420H1 Protein Lysine Methyltransferase Modulate Its Catalytic Activity. J Mol Biol 431:3068-3080 (2019). PubMed: 31255706
