Histone H4 Total Acetylation Assay Kit (Colorimetric) (ab115125)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 2 ng/well
製品の概要
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製品名
Histone H4 Total Acetylation Assay Kit (Colorimetric)
Histone H4 acetylation キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
検出感度
2 ng/well -
検出範囲
5 ng/well - 2000 ng/well -
全工程の試験時間
2h 30m -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Mammals -
製品の概要
Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 acetylation. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptional activity of eukaryotic chromatin domains. The reversible lysine acetylation of histone H4 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.
Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) enables the user to colorimetrically detect and quantify if histone H4 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) is suitable for specifically measuring total histone H4 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units Antibody Buffer 1 x 6ml 1 x 12ml Color Developer 1 x 5ml 1 x 10ml Detection Antibody, 1 mg/ml 1 x 5µl 1 x 10µl Signal Enhancer 1 x 120µl 1 x 240µl Signal Report Solution 1 x 5µl 1 x 10µl Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml -
研究分野
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab115125 は 4 報の論文で使用されています。
- Sibilia V et al. Sex Steroid Regulation of Oxidative Stress in Bone Cells: An In Vitro Study. Int J Environ Res Public Health 18:N/A (2021). PubMed: 34831936
- Casati L et al. Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration. Int J Mol Sci 21:N/A (2020). PubMed: 32629979
- Zhang D et al. miR-185 mediates lung epithelial cell death after oxidative stress. Am J Physiol Lung Cell Mol Physiol 310:L700-10 (2016). PubMed: 26747785
- Kocic G et al. Global and specific histone acetylation pattern in patients with Balkan endemic nephropathy, a worldwide disease. Ren Fail 36:1078-82 (2014). Functional Studies ; Human . PubMed: 24845033