Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4~15%)
Sample
Human Cell lysate - whole cell (Human HeLa cells)
Specification
Human HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Concentration
0.5 µg/ml
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS with 5% BSA and 0.1% Triton-X 100
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Super Signal
Bands
Specific: 15 kDa Non-specific: 49 kDa
Exposure
30 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 11 2013