Application
Western blot
Sample
Human Cell lysate - nuclear (Nuclei prepared by tri-choloro acetic acid (TCA) p)
Gel Running Conditions
Reduced Denaturing (15% BIS-TRIS gel)
Loading amount
10 µg
Specification
Nuclei prepared by tri-choloro acetic acid (TCA) p
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST buffer with 0.1% Tween-20
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Immun-Star Goat Anti-Rabbit (GAR)-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
BioRad Clarity Western ECL Blotting subs
Negative control
Used as a negative control to confirm that methylation activity of PR-SET7 is specific to H4K20 monomethylation.
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 15 kDa
Additional data
Additional Notes
The antibody is weak and requires longer exposure times to visualize the bands.
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投稿 Aug 23 2018