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  1. Link

    histone-h4-antibody-chip-grade-ab7311.pdf

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Epigenetics and Nuclear Signaling ChIP assays ChIP antibodies
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Anti-Histone H4 抗体 - ChIP Grade (ab7311)

  • Datasheet
  • SDS
Reviews (12)Q&A (34)References (92)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

ChIP - Anti-Histone H4 antibody - ChIP Grade (ab7311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody - ChIP Grade (ab7311)
  • Western blot - Anti-Histone H4 antibody - ChIP Grade (ab7311)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody - ChIP Grade (ab7311)
  • Western blot - Anti-Histone H4 antibody - ChIP Grade (ab7311)

Key features and details

  • Rabbit polyclonal to Histone H4 - ChIP Grade
  • Suitable for: IHC-P, ICC/IF, ChIP, WB
  • Reacts with: Mouse, Cow, Human, Saccharomyces cerevisiae
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Histone H4 antibody [EPR16599] - ChIP Grade (ab177840)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Histone H4 antibody - ChIP Grade
    Histone H4 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Histone H4 - ChIP Grade
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-P, ICC/IF, ChIP, WBmore details
  • 種交差性

    交差種: Mouse, Cow, Human, Saccharomyces cerevisiae
    交差が予測される動物種: Mammals
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • Calf Thymus Histone Preparation; Hela whole cell extract
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

関連製品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Histone H4 protein (ab198115)
  • Related Products

    • Human Histone H4 peptide (ab13843)
    • Human Histone H4 (symmetric di methyl R3) peptide (ab14791)
    • Histone H4 Total Quantification Kit (Colorimetric) (ab156909)
    • Human Histone H4 (tri methyl K20) peptide (ab17567)
    • Histone H4 Modification Multiplex Assay Kit (Colorimetric) (ab185914)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7311の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF (2)
1/200.
ChIP (4)
Use 2 µg for 25 µg of chromatin.
WB (5)
1/500 - 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
特記事項
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/200.
ChIP
Use 2 µg for 25 µg of chromatin.
WB
1/500 - 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).

ターゲット情報

  • 機能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 配列類似性

    Belongs to the histone H4 family.
  • 翻訳後修飾

    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • 細胞内局在

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P62805 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 121504 Human
    • Entrez Gene: 554313 Human
    • Entrez Gene: 8294 Human
    • Entrez Gene: 8359 Human
    • Entrez Gene: 8360 Human
    • Entrez Gene: 8361 Human
    • Entrez Gene: 8362 Human
    • Entrez Gene: 8363 Human
    • Entrez Gene: 8364 Human
    • Entrez Gene: 8365 Human
    • Entrez Gene: 8366 Human
    • Entrez Gene: 8367 Human
    • Entrez Gene: 8368 Human
    • Entrez Gene: 8370 Human
    • Entrez Gene: 100041230 Mouse
    • Entrez Gene: 319155 Mouse
    • Entrez Gene: 319156 Mouse
    • Entrez Gene: 319157 Mouse
    • Entrez Gene: 319158 Mouse
    • Entrez Gene: 319159 Mouse
    • Entrez Gene: 319160 Mouse
    • Entrez Gene: 319161 Mouse
    • Entrez Gene: 320332 Mouse
    • Entrez Gene: 326619 Mouse
    • Entrez Gene: 326620 Mouse
    • Entrez Gene: 674678 Mouse
    • Entrez Gene: 69386 Mouse
    • Entrez Gene: 97122 Mouse
    • Omim: 142750 Human
    • Omim: 602822 Human
    • Omim: 602823 Human
    • Omim: 602824 Human
    • Omim: 602825 Human
    • Omim: 602826 Human
    • Omim: 602827 Human
    • Omim: 602828 Human
    • Omim: 602829 Human
    • Omim: 602830 Human
    • Omim: 602831 Human
    • Omim: 602833 Human
    • SwissProt: P62805 Human
    • SwissProt: P62806 Mouse
    • SwissProt: P02309 Saccharomyces cerevisiae
    • Unigene: 143080 Human
    • Unigene: 247816 Human
    • Unigene: 248172 Human
    • Unigene: 248178 Human
    • Unigene: 248179 Human
    • Unigene: 278483 Human
    • Unigene: 46423 Human
    • Unigene: 528055 Human
    • Unigene: 533295 Human
    • Unigene: 55468 Human
    • Unigene: 591790 Human
    • Unigene: 655235 Human
    • Unigene: 662174 Human
    • Unigene: 706635 Human
    • Unigene: 718935 Human
    • Unigene: 144300 Mouse
    • Unigene: 14775 Mouse
    • Unigene: 158272 Mouse
    • Unigene: 227295 Mouse
    • Unigene: 228709 Mouse
    • Unigene: 246720 Mouse
    • Unigene: 255646 Mouse
    • Unigene: 260530 Mouse
    • Unigene: 261642 Mouse
    • Unigene: 261662 Mouse
    • Unigene: 261664 Mouse
    • Unigene: 377875 Mouse
    • Unigene: 442307 Mouse
    see all
  • 別名

    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4.k antibody
    • H4/a antibody
    • H4/b antibody
    • H4/c antibody
    • H4/d antibody
    • H4/e antibody
    • H4/g antibody
    • H4/h antibody
    • H4/I antibody
    • H4/j antibody
    • H4/k antibody
    • H4/m antibody
    • H4/n antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • H4FM antibody
    • H4FN antibody
    • H4M antibody
    • HIST1H4A antibody
    • HIST1H4B antibody
    • HIST1H4C antibody
    • HIST1H4D antibody
    • HIST1H4E antibody
    • HIST1H4F antibody
    • HIST1H4H antibody
    • HIST1H4I antibody
    • HIST1H4J antibody
    • HIST1H4K antibody
    • HIST1H4L antibody
    • HIST2H4 antibody
    • HIST2H4A antibody
    • Hist4h4 antibody
    • Histone 1 H4a antibody
    • Histone 1 H4b antibody
    • Histone 1 H4c antibody
    • Histone 1 H4d antibody
    • Histone 1 H4e antibody
    • Histone 1 H4f antibody
    • Histone 1 H4h antibody
    • Histone 1 H4i antibody
    • Histone 1 H4j antibody
    • Histone 1 H4k antibody
    • Histone 1 H4l antibody
    • Histone 2 H4a antibody
    • histone 4 H4 antibody
    • Histone H4 antibody
    • MGC24116 antibody
    see all

画像

  • ChIP - Anti-Histone H4 antibody - ChIP Grade (ab7311)
    ChIP - Anti-Histone H4 antibody - ChIP Grade (ab7311)

    Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab7311 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody - ChIP Grade (ab7311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody - ChIP Grade (ab7311)
    IHC image of Histone H4 staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7311, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blot - Anti-Histone H4 antibody - ChIP Grade (ab7311)
    Western blot - Anti-Histone H4 antibody - ChIP Grade (ab7311)
    All lanes : Anti-Histone H4 antibody - ChIP Grade (ab7311) at 1/500 dilution

    Lane 1 : Calf thymus histone extract
    Lane 2 : Calf thymus histone extract with Human Histone H4 peptide (ab21044) at 1 µg/ml
    Lane 3 : Calf thymus histone extract with Histone H4 peptide - acetyl K16 (ab22376) at 1 µg/ml
    Lane 4 : Calf thymus histone extract with Human Histone H4 (acetyl K20) peptide (ab20632) at 1 µg/ml

    Lysates/proteins at 0.5 µg per lane.

    Secondary
    All lanes : Alexa-Fluor Goat anti-rabbit IgG at 1/10000 dilution

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody - ChIP Grade (ab7311)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody - ChIP Grade (ab7311)This image is courtesy of Darin McDonald

    SKN-SH cells were fixed in 4% paraformaldehyde for 10 mins, permeabilized in PBS-0.5% Triton X-100 for 5 mins and incubated for 30 minutes with ab7311 (1/200 dilution). The slides were rinsed once in PBS-Triton (0.1%), twice in PBS then incubated with the secondary antibody for 30 mins. The DNA is stained with DAPI (blue). Clear nuclear staining with ab7311 can be seen (green).

  • Western blot - Anti-Histone H4 antibody - ChIP Grade (ab7311)
    Western blot - Anti-Histone H4 antibody - ChIP Grade (ab7311)This image is courtesy of an Abreview submitted by Rodrigo Alves.
    Anti-Histone H4 antibody - ChIP Grade (ab7311) at 1/500 dilution + Human Bone Whole Tissue Lysate at 25 µg with BSA, 1 Hour, 23°C at 5 %

    Developed using the ECL technique.

    Predicted band size: 11 kDa


    Exposure time: 1 minute

    See Abreview

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (92)

ab7311 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7311 は 92 報の論文で使用されています。

  • Tapias A  et al. HAT cofactor TRRAP modulates microtubule dynamics via SP1 signaling to prevent neurodegeneration. Elife 10:N/A (2021). PubMed: 33594975
  • Boltengagen M  et al. Dynamics of SAS-I mediated H4 K16 acetylation during DNA replication in yeast. PLoS One 16:e0251660 (2021). PubMed: 34014972
  • Lunazzi G  et al. NFAT5 Amplifies Antipathogen Responses by Enhancing Chromatin Accessibility, H3K27 Demethylation, and Transcription Factor Recruitment. J Immunol 206:2652-2667 (2021). PubMed: 34031145
  • Kim C  et al. Histone deficiency and accelerated replication stress in T cell aging. J Clin Invest 131:N/A (2021). PubMed: 34060486
  • Cheng X  et al. NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination. PLoS Genet 17:e1009459 (2021). PubMed: 34228704
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 46 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H4 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (CRC cell line)
Permeabilization
No
Specification
CRC cell line
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: 20°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

投稿 Oct 08 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H4 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
Yes - PBS, Tween
Specification
Colon cancer
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Aug 16 2021

ChIP abreview for Anti-Histone H4 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Mouse Tissue lysate - nuclear (Hippocampus)
Negative control
Normal rabbit IgG
Specification
Hippocampus
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde in PBS
Positive control
One active gene (ActB) and one repressed gene (HoxA1). The ChIP show higher levels of H4 in the repressed gene.
Read More

Abcam user community

Verified customer

投稿 Sep 06 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H4 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Kyoto)
Permeabilization
No
Specification
HeLa Kyoto
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Methanol
Read More

DR. Andrew Bowman

Verified customer

投稿 Apr 21 2017

Western blot abreview for Anti-Histone H4 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (multiple myeloma cell)
Loading amount
50 µg
Specification
multiple myeloma cell
Gel Running Conditions
Reduced Denaturing (10-20% tris glycine gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 Nov 21 2012

Western blot abreview for Anti-Histone H4 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Bone tissue)
Loading amount
25 µg
Specification
Bone tissue
Gel Running Conditions
Reduced Denaturing (15% Gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Dr. Rodrigo Alves

Verified customer

投稿 Apr 17 2012

Question

Hello,



Thanks so much! I tried your two product two times but however I increase [c] or incubation I still can not get any right sizebands. Do you have any positive cell lysate available for me to confirm the antibody. I really need technical support on myb ab since other antibodies (P53, P27, PARP and etc.)works easily in my hands.

Read More

Abcam community

Verified customer

Asked on Jun 05 2012

Answer

Thanks in advance for filling out the questionnaire below for each antibody. The answers will help me to assist you most efficiently.

1) Abcam product code ab



2) Abcam order reference number or product batch number



3) Description of the problem



4) Sample preparation:

Type of sample (whole cell lysates, fraction, recombinant protein…):

Lysis buffer

Protease inhibitors:

Phosphatase inhibitors

Reducing agent

Boiling for ≥5 min? yes/no

Protein loaded ug/lane or cells/lane

Positive control

Negative control



5) Percentage of gel

Type of membrane

Protein transfer verified

Blocking agent and concentration

Blocking time

Blocking temperature



6) Primary antibody (If more than one was used, describe in “additional notes”) :

Concentration or dilution

Diluent buffer

Incubation time

Incubation temperature:



7) Secondary antibody:

Species:

Reacts against:

Concentration or dilution

Diluent buffer

Incubation time

Incubation temperature:

Fluorochrome or enzyme conjugate:



8) Washing after primary and secondary antibodies:

Buffer

Number of washes



9)Detection method

10) How many times have you run this staining?

Do you obtain the same results every time?

What steps have you altered to try and optimize the use of this antibody?



Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem.





Please send this questionnaire by e-mail by cutting and pasting the text in your e-mail (no attachments other than images) to technical@abcam.com.

Read More

Abcam Scientific Support

Answered on Jun 05 2012

Question

Hello,



My abcam order still doesnot arrive.


card10 ab ab64171.



Thanks.

Read More

Abcam community

Verified customer

Asked on Jun 05 2012

Answer

Thanks for your email.

This item is currently back ordered 1-2 weeks from date the order is placed. You should expect to receive this vial of ab64171 in approximately 1 week.

I hope this is helpful. Please contact us again if you have any further questions.

Read More

Abcam Scientific Support

Answered on Jun 05 2012

Question

Thanks so much for your previous help!



I just got two antibodies and I will test them: ab116018 and ab7291. If they have problem I will contact with you later. Best,

Read More

Abcam community

Verified customer

Asked on Apr 26 2012

Answer

Fantastic! Best of luck in your experiments!

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Abcam Scientific Support

Answered on Apr 26 2012

Question

I am trying to order H4 total acetylation Ab from your place. But I can not find it.

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Abcam community

Verified customer

Asked on Apr 17 2012

Answer

Thank you for your enquiry.

We have a number of different antibodies against specific acetylated residues on histone H4. However an antibody does not exist that will detect every acetylated residue on histone H4.

You may consider just using one or two antibodies against commonly acetylated histone H4 residues. I'm sure this information is available in the literature.

I hope this is helpful. Please contact me again if you have any further questions.

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Abcam Scientific Support

Answered on Apr 17 2012

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