Anti-Histone H4 抗体 (ab16483)
Key features and details
- Rabbit polyclonal to Histone H4
- Suitable for: WB
- Reacts with: Cow, Human, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-Histone H4 antibody
Histone H4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Histone H4 -
由来種
Rabbit -
特異性
We recommend ab16483 as a suitable alternative to ab10156 as it was made using the same immunogen. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Cow, Human, Recombinant fragment
交差が予測される動物種: Saccharomyces cerevisiae
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免疫原
Recombinant full length protein corresponding to Saccharomyces cerevisiae Histone H4 aa 1 to the C-terminus.
Database link: P02309 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16483の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa). |
ターゲット情報
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機能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
配列類似性
Belongs to the histone H4 family. -
翻訳後修飾
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
別名
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
画像
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Western blot - Anti-Histone H4 antibody (ab16483)All lanes : Anti-Histone H4 antibody (ab16483) at 1 µg/ml
Lane 1 : Calf Thymus Histone lysate at 0.5 µg
Lane 2 : HeLa Histone Lysate at 2.5 µg
Lane 3 : Recombinant Histone H4 at 1 µg
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 11 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?ab16483 detects a band of approximately 14 kDa in Calf thymus histone prep, HeLa histone prep and recombinant Histone H4. This is slightly larger than the predicted molecular weight according to Swissprot (11 kDa), however we are confident that ab16483 is recognising Histone H4.
データシートおよび資料
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Datasheet download
参考文献 (14)
ab16483 は 14 報の論文で使用されています。
- Bhattacharjee P et al. Epigenetic regulations in alternative telomere lengthening: Understanding the mechanistic insight in arsenic-induced skin cancer patients. Sci Total Environ 704:135388 (2020). PubMed: 31837846
- Koziol K et al. Changes in ?H2AX and H4K16ac levels are involved in the biochemical response to a competitive soccer match in adolescent players. Sci Rep 10:14481 (2020). PubMed: 32879387
- Selvarajah B et al. mTORC1 amplifies the ATF4-dependent de novo serine-glycine pathway to supply glycine during TGF-ß1-induced collagen biosynthesis. Sci Signal 12:N/A (2019). PubMed: 31113850
- Vadla R & Haldar D Mammalian target of rapamycin complex 2 (mTORC2) controls glycolytic gene expression by regulating Histone H3 Lysine 56 acetylation. Cell Cycle 17:110-123 (2018). PubMed: 29143563
- Khan MA et al. Regulating NETosis: Increasing pH Promotes NADPH Oxidase-Dependent NETosis. Front Med (Lausanne) 5:19 (2018). PubMed: 29487850
