Anti-Histone H3 (tri methyl K4) 抗体 [mAbcam1012] - ChIP Grade (ab1012)

Reviews (21) Submit a question References (181)
ChIP - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
  • Immunocytochemistry - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
  • ELISA - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
  • Peptide Array - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
  • Western blot - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
  • Immunocytochemistry - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
  • Flow Cytometry - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)

Key features and details

  • Mouse monoclonal [mAbcam1012] to Histone H3 (tri methyl K4) - ChIP Grade
  • Suitable for: PepArr, ELISA, ChIP, Flow Cyt, WB, ICC
  • Reacts with: Rat, Cow, Human, Rice, Recombinant fragment
  • Isotype: IgG2b

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647

製品の概要

  • 製品名

    Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade
    Histone H3 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [mAbcam1012] to Histone H3 (tri methyl K4) - ChIP Grade

  • 由来種

    Mouse

  • 特異性

    By ELISA the antibody binds to the tri methyl K4 peptide and partially to di and mono methyl K4 peptides (there is <10% cross reactivity with di methyl K4). It does not bind to unmodified, mono, di or tri methyl K9 or di or tri methyl K27 peptides. Not suitable for blocking with milk in Western blot (see Application notes).

  • アプリケーション

    適用あり: PepArr, ELISA, ChIP, Flow Cyt, WB, ICCmore details

  • 種交差性

    交差種: Rat, Cow, Human, Rice, Recombinant fragment
    交差が予測される動物種: Mouse, Sheep, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe, Zebrafish, Mammals

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab1342)

  • ポジティブ･コントロール

    • ChIP: GAPDH primer pair ab267832. ICC: HeLa cells.

  • 特記事項

    Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    mAbcam1012

  • アイソタイプ

    IgG2b

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab1012の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
PepArr
Use a concentration of 0.5 - 2 µg/ml.
ELISA
Use at an assay dependent concentration.
ChIP (9)
Use 2-5 µg for 25 µg of chromatin.

Use GAPDH ChIP primer pair ab267832 as positive control.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB (7)
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human Histone H3 (tri methyl K4) peptide (ab1342). NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have investigated the blocking conditions for this antibody and found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see image below).
ICC
Use a concentration of 5 µg/ml.
特記事項
PepArr
Use a concentration of 0.5 - 2 µg/ml.
ELISA
Use at an assay dependent concentration.
ChIP
Use 2-5 µg for 25 µg of chromatin.

Use GAPDH ChIP primer pair ab267832 as positive control.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human Histone H3 (tri methyl K4) peptide (ab1342). NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have investigated the blocking conditions for this antibody and found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see image below).
ICC
Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

  • 配列類似性

    Belongs to the histone H3 family.

  • 発生段階

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

  • 翻訳後修飾

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.

  • 細胞内局在

    Nucleus. Chromosome.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

画像

  • ChIP - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
    ChIP - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)

    Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25 µg of chromatin, 5 µg of  ab1012 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • Immunocytochemistry - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
    Immunocytochemistry - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)

    ab1012 staining Histone H3 (tri methyl K4) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab1012 at 5 µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • ELISA - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
    ELISA - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)

    ELISA using ab1012 at varying antibody concentrations.

    Curve_SPL1 indicates binding to the Human Histone H3 (tri methyl K4) peptide (ab1342). Curve_SPL4 indicates partial binding to the Human Histone H3 (di methyl K4) peptide (ab7768). There is very weak cross-reactivity with the Human Histone H3 (mono methyl K4) peptide (ab1340) (Curve_SPL3).

    Binding to the following peptides was not seen: SPL2 unmodified Histone H3, SPL5 Histone H3 mono methyl K9, SPL6 Histone H3 di methyl K9, SPL7 Histone H3 tri methyl K9, SPL8 Histone H3 mono methyl K27, SPL9 Histone H3 di methyl K27, SPL10 Histone H3 tri methyl K27.

  • Peptide Array - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
    Peptide Array - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)

    All batches of ab1012 are tested in Peptide Array against peptides to different Histone modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - tri methyl K4 peptide (ab92374), indicating that this antibody specifically recognises the Histone H3 - tri methyl K4 modification.

    ab92374 - Histone H3 - tri methyl K4
    ab7768 - Histone H3 - di methyl K4
    ab179150 - Histone H3 - asymmetric di methyl R2
    ab179142 - Histone H3 - mono methyl R2
    ab1784 - Histone H3 - di methyl K36
    ab179146 - Histone H3 - symmetric di methyl R2
    ab36927 - Histone H2A - phospho S122
    ab46854 - Histone H3 - tri methyl K18
    ab179021 - Histone H2A - symmetric di methyl R29
    ab17632 - Histone H4 - biotinylated K5
    ab179159 - Histone H3 - phospho T3
    ab14103 - Histone H3 - phopsho T6
    ab1783 - Histone H3 - mono methyl K36
    ab179177 - Histone H3 - mono methyl K4 + Histone H3 di methyl K9
  • Western blot - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
    Western blot - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
    Lane 1 : Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012) at 1 µg/ml (Blocked in 5% BSA)
    Lane 2 : Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012) at 1 µg/ml (Blocked in 5% MILK)

    All lanes : Calf Thymus Histone Preparation Nuclear Lysate

    Lysates/proteins at 0.5 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 10 minutes
  • Immunocytochemistry - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
    Immunocytochemistry - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)This image is courtesy of Darin McDonald, Cross Cancer Institute
    SK-N-SK cells were fixed in 4% paraformaldehdye, permeabilized in 0.5% Triton X-100 and incubated with ab1012 (1/100). The antibody clearly stains the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification.
  • Flow Cytometry - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
    Flow Cytometry - Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)

    Overlay histogram showing HeLa cells stained with ab1012 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab1012, 1µg/1x106) for 30 min at 22°C.  The secondary antibody used was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.

    Isotype control antibody (black line) was Mouse IgG2b [7E10G10] isotype control (ab170192) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

参考文献 (181)

ab1012 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1012 は 181 報の論文で使用されています。

  • Ferraro AR  et al. Chromatin accessibility profiling in Neurospora crassa reveals molecular features associated with accessible and inaccessible chromatin. BMC Genomics 22:459 (2021). PubMed: 34147068
  • Kim CY  et al. HOXA5 confers tamoxifen resistance via the PI3K/AKT signaling pathway in ER-positive breast cancer. J Cancer 12:4626-4637 (2021). PubMed: 34149926
  • Yang W  et al. Cyclophosphamide Exposure Causes Long-Term Detrimental Effect of Oocytes Developmental Competence Through Affecting the Epigenetic Modification and Maternal Factors' Transcription During Oocyte Growth. Front Cell Dev Biol 9:682060 (2021). PubMed: 34164401
  • Xiong Y  et al. Bisphenol a affects endometrial stromal cells decidualization, involvement of epigenetic regulation. J Steroid Biochem Mol Biol 200:105640 (2020). PubMed: 32087250
  • Yang X  et al. Long Noncoding RNA AW112010 Promotes the Differentiation of Inflammatory T Cells by Suppressing IL-10 Expression through Histone Demethylation. J Immunol 205:987-993 (2020). PubMed: 32690657
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 21 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (CRC cell line)
Permeabilization
Yes - PBS-Tween
Specification
CRC cell line
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

投稿 Jul 23 2021

Western blot abreview for Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Mammary cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
1e+006 cells
Specification
Mammary cell line
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Feb 28 2017

ChIP abreview for Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - whole cell (Gut)
Specification
Gut
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Sep 26 2016

ChIP abreview for Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
ChIP
Detection step
Real-time PCR
Sample
Human Cell lysate - nuclear (Breast)
Specification
Breast
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Sep 02 2014

Western blot abreview for Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (Breast)
Specification
Breast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

投稿 Sep 01 2014

Western blot abreview for Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Colon)
Specification
Colon
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

投稿 Sep 01 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (methylated) antibody [mAbcam1012] - ChIP Grade

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SAOS-2 human osteosarcoma)
Specification
SAOS-2 human osteosarcoma
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS-triton .25%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Apr 19 2012

ChIP abreview for Anti-Histone H3 (methylated) antibody [mAbcam1012] - ChIP Grade

 Good
Abreviews
Application
ChIP
Sample
Human Cell lysate - nuclear (Colon)
Specification
Colon
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Real-time PCR
Positive control
Mouse anti-cmyc
Negative control
BSA
Read More

Abcam user community

Verified customer

投稿 Aug 19 2011

Western blot abreview for Anti-Histone H3 (methylated) antibody [mAbcam1012] - ChIP Grade

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (lysate prepared from 1 OD of cells from liquid cul)
Loading amount
17 µg
Specification
lysate prepared from 1 OD of cells from liquid cul
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More

DR. Marc Meneghini

Verified customer

投稿 Apr 20 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (methylated) antibody [mAbcam1012] - ChIP Grade

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
Yes - Triton X100 0.1%
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Read More

Dr. Chantal Desmaze

Verified customer

投稿 Jan 07 2011

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com