アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報
  1. Link

    histone-h3-tri-methyl-k4-antibody-epr20551-225-chip-grade-ab213224.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Histones H3 Methylated
Share by email
リコンビナントRabMAb

Anti-Histone H3 (tri methyl K4) 抗体 [EPR20551-225] - ChIP Grade (ab213224)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question References (5)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

ChIP-sequencing - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
  • ChIP - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
  • ChIP-sequencing - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
  • Western blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
  • Western blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
  • Peptide Array - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
  • Immunoprecipitation - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
  • Dot Blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
  • Flow Cytometry - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
  • Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20551-225] to Histone H3 (tri methyl K4) - ChIP Grade
  • Suitable for: ChIP-sequencing, ChIP, Flow Cyt, WB, ICC/IF, Dot blot, PepArr, IP
  • Reacts with: Mouse, Rat, Human

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
タンパク質
Product image
Recombinant human Histone H3 protein (Active) (ab198757)

関連製品

製品の概要

  • 製品名

    Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade
    Histone H3 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR20551-225] to Histone H3 (tri methyl K4) - ChIP Grade
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ChIP-sequencing, ChIP, Flow Cyt, WB, ICC/IF, Dot blot, PepArr, IPmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Histone H3 aa 1-100 (tri methyl K4). The exact sequence is proprietary.
    Database link: P68431

  • ポジティブ・コントロール

    • WB: HeLa, NIH/3T3 and C6 whole cell lysates. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt: HeLa cells. IP: NIH/3T3 whole cell lysate. ChIP: Chromatin prepared from HeLa cells. ChIP-seq: Chromatin prepared from HeLa cells.
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR20551-225
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

関連製品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] (ab237340)
    • Alexa Fluor® 647 Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] (ab237341)
    • PE Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] (ab237342)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

アプリケーション

Our Abpromise guarantee covers the use of ab213224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP-sequencing Use 4 µg for 30 µg of chromatin.
ChIP Use 2 µg for 25 µg of chromatin.
Flow Cyt 1/500.
WB 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

We recommend to use 2% BSA as blocking and antibody dilution buffer.

ICC/IF 1/500.
Dot blot 1/1000.
PepArr Use a concentration of 0.1 µg/ml.
IP 1/30.

ターゲット情報

  • 機能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 配列類似性

    Belongs to the histone H3 family.
  • 発生段階

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • 翻訳後修飾

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • 細胞内局在

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Entrez Gene: 100364501 Rat
    • Entrez Gene: 100365669 Rat
    • Entrez Gene: 291159 Rat
    • Entrez Gene: 679994 Rat
    • Entrez Gene: 680511 Rat
    • Entrez Gene: 682330 Rat
    • Omim: 602810 Human
    • SwissProt: P68431 Human
    • SwissProt: P68433 Mouse
    • SwissProt: Q6LED0 Rat
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 397328 Mouse
    • Unigene: 138090 Rat
    see all
  • 別名

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

画像

  • ChIP-sequencing - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    ChIP-sequencing - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 4 µg of Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

    Additional screenshots of mapped reads can be downloaded here.

  • ChIP - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    ChIP - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

    Chromatin was prepared from Hela (human epithelial cel line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 2 μg of ab213224(red), and 20 μl of Anti-rabbit IgG sepharose beads. 2 μg of Rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • ChIP-sequencing - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    ChIP-sequencing - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed by Active Motif, Carlsbad, CA.

    Additional screenshots of mapped reads can be downloaded here.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1%, Triton X-100-permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling Histone H3 (tri methyl K4) with ab213224 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining on NIH/3T3 cell lines.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    Western blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    All lanes : Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) at 1/5000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 3 : C6 (rat glial tumor cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% BSA/TBST.

  • Western blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    Western blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    All lanes : Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) at 1/5000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 2% BSA/TBST

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 15 kDa


    Exposure time: 40 seconds


    We recommend to use 2% BSA as blocking and antibody dilution buffer.

  • Peptide Array - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    Peptide Array - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

    ab213224 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

  • Immunoprecipitation - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    Immunoprecipitation - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

    Histone H3 (tri methyl K4) was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) lysate with ab213224 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213224 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution

    Lane 1: NIH/3T3 whole cell lysate 10 µg (Input). 

    Lane 2: ab213224 IP in NIH/3T3 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213224 in NIH/3T3 whole cell lysate.

     

    Exposure time: 3 minutes.

    Blocking and dilution buffer and concentration: 5% BSA/TBST.

  • Dot Blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    Dot Blot - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

    Dot blot analysis of Histone H3 (tri methyl K4) labeled with ab213224 at 1/1000 dilution.

    Lane 1: Histone H3K4Me3 peptide.

    Lane 2: Histone H3 unmodified peptide.

    Lane 3: Histone H3K(18+K36)Me3 peptide.

    Lane 4: Histone H3K18Me3 peptide.

    Lane 5: Histone H3K4Me2 peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

     

    Exposure time: 3 minutes.

    Blocking and dilution buffer: 5% NFDM/TBST.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (tri methyl K4) with ab213224 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive nuclear staining on HeLa cell line. 

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    Flow Cytometry - Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Histone H3 (tri methyl K4) with ab213224 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)
    Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • 参考文献 (5)

    ab213224 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab213224 は 5 報の論文で使用されています。

    • Wong M  et al. JMJD6 is a tumorigenic factor and therapeutic target in neuroblastoma. Nat Commun 10:3319 (2019). PubMed: 31346162
    • Huang H  et al. CRISPR/dCas9-mediated activation of multiple endogenous target genes directly converts human foreskin fibroblasts into Leydig-like cells. J Cell Mol Med 23:6072-6084 (2019). PubMed: 31264792
    • Ngwa CJ  et al. The G9a Histone Methyltransferase Inhibitor BIX-01294 Modulates Gene Expression during Plasmodium falciparum Gametocyte Development and Transmission. Int J Mol Sci 20:N/A (2019). PubMed: 31615031
    • Zhang XD  et al. The interaction of lncRNA EZR-AS1 with SMYD3 maintains overexpression of EZR in ESCC cells. Nucleic Acids Res 46:1793-1809 (2018). PubMed: 29253179
    • Yang L  et al. KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system. EMBO Rep 19:N/A (2018). PubMed: 30389724

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    ChIP abreview for Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (T-cell lymphoma (TLBR-2))
    Negative control
    Standard negative control (alpha-satellite region)
    Specification
    T-cell lymphoma (TLBR-2)
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Positive control
    Promoter region of a published target (10.1038/s41375-020-0754-8)
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 02 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    ブログ

    ブログを読む

    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2021 Abcam plc. All rights reserved.