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  1. Link

    histone-h3-tri-methyl-k4-antibody-chip-grade-ab8580.pdf

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Epigenetics and Nuclear Signaling Histones H3 Methylated
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Anti-Histone H3 (tri methyl K4) 抗体 - ChIP Grade (ab8580)

  • Datasheet
  • SDS
Reviews (81)Q&A (23)References (1513)

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ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
  • Western blot - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
  • Peptide Array - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
  • ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)

Key features and details

  • Rabbit polyclonal to Histone H3 (tri methyl K4) - ChIP Grade
  • Suitable for: PepArr, ChIP, WB, IHC-P, ICC/IF
  • Reacts with: Cow, Human
  • Isotype: IgG

こちらの製品もご検討ください

Biochemical
GSK-J1, H3K27 histone demethylase inhibitor (ab144396)
一次抗体
Product image
Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (ab1791)

関連製品

このターゲットにつきましては、リコンビナント・ウサギ・モノクローナル抗体も販売しています

製品の概要

  • 製品名

    Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade
    Histone H3 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Histone H3 (tri methyl K4) - ChIP Grade
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: PepArr, ChIP, WB, IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Cow, Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Pig, Saccharomyces cerevisiae, Tetrahymena, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Indian muntjac, Oikopleura, Plants, Zebrafish, Mammals, Trypanosoma cruzi, Common marmoset, Rice, Xenopus tropicalis
  • 免疫原

    Synthetic peptide within Human Histone H3 aa 1-100 (tri methyl K4) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab92374)

  • 特記事項

    In immunofluorescence, a distinct property of tri methyl lysine 4 is its apparent 'ringing' of regions that appear as nucleoplasmic 'holes'. These represent the positions of splicing factor compartments, which often are easy to identify using only DNA stains in Indian muntjac fibroblasts. These splicing factor compartments are known to be preferentially associated with active genes and highly acetylated histone H3. This antibody, as expected, fails to stain heterochromatin (work by Kirk McManus, lab of Michael Hendzel).

    The immunofluorescence results suggest this antibody is an exceptional euchromatin probe.

    Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). See other anti-rabbit secondary antibodies that can be used with this antibody.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

関連製品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Control Peptide

    • Human Histone H3 (tri methyl K4) peptide (ab1342)
    • Human Histone H3 (unmodified) peptide (ab7228)
  • Immunizing Peptide (Blocking)

    • Human Histone H3 (tri methyl K4) peptide (ab92374)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • GAPDH positive control ChIP primer pair (ab267832)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)
  • Related Products

    • Anti-Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)
    • Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452)
    • Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455)
    • Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056)
    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)
    • Human Histone H3 (tri methyl K9) peptide (ab1773)
    • Human Histone H3 (tri methyl K27) peptide (ab1782)
    • Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)
    • Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (ab7766)
    • Human Histone H3 (di methyl K4) peptide (ab7768)
    • Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895)

アプリケーション

Our Abpromise guarantee covers the use of ab8580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
PepArr Use a concentration of 0.2 - 0.02 µg/ml.

Slight cross reactivity is observed with the Histone H3 - di methyl K4 modification. Optimisation is recommended to avoid array signal saturation.

ChIP Use 2 µg for 25 µg of chromatin.

We recommend GAPDH positive control ChIP primer pair ab267832 as positive control.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

1/100 - 1/5000

ターゲット情報

  • 機能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 配列類似性

    Belongs to the histone H3 family.
  • 発生段階

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • 翻訳後修飾

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • 細胞内局在

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Entrez Gene: 100364501 Rat
    • Entrez Gene: 100365669 Rat
    • Entrez Gene: 291159 Rat
    • Entrez Gene: 679994 Rat
    • Entrez Gene: 680511 Rat
    • Entrez Gene: 682330 Rat
    • Omim: 602810 Human
    • SwissProt: P68432 Cow
    • SwissProt: P68431 Human
    • SwissProt: P68433 Mouse
    • SwissProt: Q6LED0 Rat
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 397328 Mouse
    • Unigene: 138090 Rat
    see all
  • 別名

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

画像

  • ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
    ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)

    Chromatin was prepared from U-2 OS (Human bone osteosarcoma epithelial cell line) cells according to the Abcam X-ChIP protocol.

    Cells were fixed with formaldehyde for 10 minutes. The  ChIP was performed with 25 µg of chromatin, 2 µg of  ab8580 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
    Western blot - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
    Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) at 1 µg/ml + Calf thymus histone preparation (nuclear lysate) at 0.5 µg

    Secondary
    Goat Anti-Rabbit IgG (H+L) HRP- conjugated antibody at 1/50000 dilution

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)This image is courtesy of Ahmad Khalil and Daniel Driscoll, University of Florida College of Medicine.

    Human female lymphoblast immunostained with ab8580 (1:100) (yellowish green) specific for histone H3 lysine 4 (H3-K4) trimethylation; the DNA is stained red with propidium iodide (PI).

    Note the inactive X chromosome (arrow) and pericentromeric heterochromatin are largely devoid of this modification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)

    IHC image of ab8580 staining Histone H3 (tri methyl K4) in human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab8580, 1/500 dilution, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    No primary antibody was used in the negative control (inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)This image is courtesy of an Abreview submitted by Dr Eva Bartova

    ab8580 staining cultured human primary fibroblasts by ICC.

    Cells were fixed in PFA and permeabilized in Triton X-100 and saponin prior to blocking with 1% BSA for 1 hour at RT.  The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C.  An FITC-conjugated rabbit anti-rabbit IgG antibody was used as the secondary.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)

    ab8580 staining Histone H3 (tri methyl K4) in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    All cells were fixed with 100% methanol (5 minutes) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1 hour. The cells were then incubated with ab4729 at 1/1000 and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1 hour with goat anti-rabbit Alexa-Fluor®488 secondary (ab150077) at 2 μg/ml (shown in green) and goat anti-mouse Alexa-Fluor®594 secondary (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labeled in blue with DAPI.

    Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no non-specific reaction between primary and secondary antibodies used.

  • Peptide Array - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
    Peptide Array - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)

    All batches of ab8580 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - tri methyl K4 peptide (ab1342), indicating that this antibody specifically recognises the Histone H3 - tri methyl K4 modification. Slight cross reactivity is observed with the Histone H3 - di methyl K4 modification. Optimization is recommended to avoid array signal saturation.

    1. ab1340 - Histone H3 - mono methyl K4
    2. ab1342 - Histone H3 - tri methyl K4
    3. ab1771 - Histone H3 - mono methyl K9
    4. ab1772 - Histone H3 - di methyl K9
    5. ab1773 - Histone H3 - tri methyl K9
    6. ab1780 - Histone H3 - mono methyl K27
    7. ab1781 - Histone H3 - di methyl K27
    8. ab1782 - Histone H3 - tri methyl K27
    9. ab7228 - Histone H3 - unmodified
    10. ab7768 - Histone H3 - di methyl K4
  • ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
    ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)This image is courtesy of an anonymous Abreview

    Chromatin was prepared from human cell lysate - nuclear B cells according to the Abcam X-ChIP protocol.

    Cells were fixed with formaldehyde for 10 minutes. The  ChIP was performed with 0.5 µg of ab8580 per µg chromatin in ChIP Buffer fot 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region. Negative control: IgG and Gene Desert. Positive control: GAPDH Promoter.

     

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)This image is courtesy of Kirk McManus in the lab of Michael Hendzel, University of Alberta

    Staining (green) with the anti-trimethyl Lysine K4 of Histone H3 antibody (ab8580) shows ringing of regions that appear as nucleoplasmic holes. These represent the positions of splicing factor compartments, which are preferentially associated with active genes and highly acetylated histone H3.   

    The antibody, as expected, fails to stain heterochromatin (red). 

プロトコール

  • Chromatin Immunofluorescence

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (1513)

    ab8580 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8580 は 1513 報の論文で使用されています。

    • Han M  et al. Therapeutic implications of altered cholesterol homeostasis mediated by loss of CYP46A1 in human glioblastoma. EMBO Mol Med 12:e10924 (2020). PubMed: 31777202
    • Li Q  et al. p53 Integrates Temporal WDR5 Inputs during Neuroectoderm and Mesoderm Differentiation of Mouse Embryonic Stem Cells. Cell Rep 30:465-480.e6 (2020). PubMed: 31940490
    • Domingues AF  et al. Loss of Kat2a enhances transcriptional noise and depletes acute myeloid leukemia stem-like cells. Elife 9:N/A (2020). PubMed: 31985402
    • Castelijns B  et al. Hominin-specific regulatory elements selectively emerged in oligodendrocytes and are disrupted in autism patients. Nat Commun 11:301 (2020). PubMed: 31949148
    • Karagianni P  et al. Bookmarking by Non-pioneer Transcription Factors during Liver Development Establishes Competence for Future Gene Activation. Cell Rep 30:1319-1328.e6 (2020). PubMed: 32023452
    View all Publications for this product

    レビューと Q&A

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    1-10 of 104 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (methylated) (tri methyl K4) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Marmoset (common) Tissue sections (Adult marmoset testis)
    Specification
    Adult marmoset testis
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval solution
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. 煜欽 林

    Verified customer

    投稿 Aug 28 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (methylated) (tri methyl K4) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MCF-7)
    Specification
    MCF-7
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Triton-X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 16 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (methylated) (tri methyl K4) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse ES cells)
    Specification
    mouse ES cells
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 23 2012

    Immunocytochemistry abreview for Anti-Histone H3 (methylated) (tri methyl K4) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (HeLa cells, human cervical carcinoma cells)
    Specification
    HeLa cells, human cervical carcinoma cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More

    Dr. Roman Hudec

    Verified customer

    投稿 Feb 09 2011

    Western blot abreview for Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Platynereis dumerilii Cell lysate - nuclear (Whole animals: 4d & 7m post fertilization)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    15 µg
    Specification
    Whole animals: 4d & 7m post fertilization
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Quentin Schenkelaars

    Verified customer

    投稿 Jul 01 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Lizard Tissue sections (Gonad)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Gonad
    Blocking step
    Opal Blocking Buffer as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 13 2019

    Western blot abreview for Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (17,5)
    Loading amount
    20 µg
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 03 2019

    Western blot abreview for Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Schmidtea mediterranea Cell lysate - nuclear (Whole planarian worm)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    30 µg
    Specification
    Whole planarian worm
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Anish Dattani

    Verified customer

    投稿 Feb 08 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (uterus)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    uterus
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 25 2018

    ChIP abreview for Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (HepG2)
    Negative control
    rabbit IgG (rIgG)
    Specification
    HepG2
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Positive control
    no positive control
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 07 2017

    1-10 of 104 Abreviews or Q&A

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