Histone H3 Total Acetylation Assay Kit (Fluorometric) (ab131561)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.4 ng
製品の概要
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製品名
Histone H3 Total Acetylation Assay Kit (Fluorometric)
Histone H3 acetylation キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
検出感度
> 0.4 ng -
検出範囲
0.4 ng - 50 ng -
全工程の試験時間
2h 30m -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Mammals -
製品の概要
Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. The reversible lysine acetylation of histone H3 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.
ab131561 is designed for measuring total histone H3 acetylation in a fast format. In an assay with this kit, the acetyl histone H3 is captured to the strip wells coated with an anti-acetyl histone H3 antibody. The captured acetyl histone H3 can then be detected with a labeled detection antibody followed by a fluorescent development reagent. The ratio of acetyl histone H3 is proportional to the intensity of fluorescence. The absolute amount of acetyl histone H3 can be quantified by comparing to the standard control.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 ラベル 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Assay Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips Green Ringed 2 units 3 units Antibody Buffer 1 x 6ml 1 x 12ml Detection Antibody, 1 mg/mL 1 x 5µl 1 x 10µl Fluoro Developer 1 x 12µl 1 x 24µl Fluoro Dilution 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 12µl 1 x 24µl Standard Control (100 µg/mL) 1 x 10µl 1 x 20µl -
研究分野
関連製品
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab131561 は 6 報の論文で使用されています。
- Liu CT et al. Targeting glycolysis in Th2 cells by pterostilbene attenuates clinical severities in an asthmatic mouse model and IL-4 production in peripheral blood from asthmatic patients. Immunology 166:222-237 (2022). PubMed: 35285025
- Roy S et al. Phloroglucinol Treatment Induces Transgenerational Epigenetic Inherited Resistance Against Vibrio Infections and Thermal Stress in a Brine Shrimp (Artemia franciscana) Model. Front Immunol 10:2745 (2019). PubMed: 31827471
- Jha PK et al. HAT2 mediates histone H4K4 acetylation and affects micrococcal nuclease sensitivity of chromatin in Leishmania donovani. PLoS One 12:e0177372 (2017). Leishmania . PubMed: 28486547
- Norouzitallab P et al. Probing the phenomenon of trained immunity in invertebrates during a transgenerational study, using brine shrimp Artemia as a model system. Sci Rep 6:21166 (2016). PubMed: 26876951
- Pestana JL et al. Non-lethal heat shock increases tolerance to metal exposure in brine shrimp. Environ Res 151:663-670 (2016). PubMed: 27619211
- Norouzitallab P et al. Environmental heat stress induces epigenetic transgenerational inheritance of robustness in parthenogenetic Artemia model. FASEB J 28:3552-63 (2014). Functional Studies ; Other species . PubMed: 24755740