Application
IHC - Wholemount
Sample
Zebrafish Embryo (Whole mount (whole zebrafish larvae))
Specification
Whole mount (whole zebrafish larvae)
Other product details
Dilution
1/200
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% horse serum in PBS with 0.1% Tween
Secondary antibody
Name
Non-Abcam antibody was used: Dylite 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: DyLight® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: DyLight® 488
Additional data
Additional Notes
5 day old zebrafish were permeabilised with proteinase K (10ug/ml 50min 37degrees), prior to staining.
Staining visible in mitotic cells. Image shows 2 cells labelled with H3 phospho S10 antibody (green) in the zebrafish jaw, nuclei are labelled with DAPI (blue).
Staining visible in mitotic cells. Image shows 2 cells labelled with H3 phospho S10 antibody (green) in the zebrafish jaw, nuclei are labelled with DAPI (blue).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Chrissy Hammond
Verified customer
投稿 Mar 21 2012