Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa)
Loading amount
10 µg
Specification
HeLa
Treatment
G9a specific siRNA
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS-T+0.5% milk
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG(Fc) AP conjugate
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/5000
Detection
Detection method
NBT/BCIP
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 16 kDa
Positive control
control siRNA
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 23 2009