アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報

新型コロナウイルス (COVID-19)に伴う当社の対応について

  1. Link

    histone-h3-mono-methyl-k79-antibody-chip-grade-ab2886.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Histones H3 Methylated
Share by email

Anti-Histone H3 (mono methyl K79) 抗体 - ChIP Grade (ab2886)

  • Datasheet
Reviews (10)Q&A (2)References (47)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
  • Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
  • Peptide Array - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
  • Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
  • ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
  • Immunoprecipitation - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)

Key features and details

  • Rabbit polyclonal to Histone H3 (mono methyl K79) - ChIP Grade
  • Suitable for: ChIP, IP, WB, PepArr
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG

こちらの製品もご検討ください

一次抗体
Product image
Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR18466] (ab191433)
タンパク質
Product image
Recombinant human Histone H3 protein (Active) (ab198757)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade
    Histone H3 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Histone H3 (mono methyl K79) - ChIP Grade
  • 由来種

    Rabbit
  • 特異性

    Reacts with Mono-methyl K79 of histone H3. Slight cross-reactivity to di-methyl K79.
  • アプリケーション

    適用あり: ChIP, IP, WB, PepArrmore details
  • 種交差性

    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic peptide within Human Histone H3 aa 50 to the C-terminus (mono methyl K79) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab4555)

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

関連製品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Control Peptide

    • Human Histone H3 (mono methyl K9) peptide (ab1771)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)
  • Related Products

    • Histone H3 (mono-methyl K79) Quantification Kit (Colorimetric) (ab115083)

アプリケーション

Our Abpromise guarantee covers the use of ab2886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use 2µg for 106 cells.
IP Use at an assay dependent concentration.
WB 1/1 - 1/500. Detects a band of approximately 17 kDa.
PepArr Use a concentration of 0.2 - 0.02 µg/ml.

ターゲット情報

  • 機能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 配列類似性

    Belongs to the histone H3 family.
  • 発生段階

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • 翻訳後修飾

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • 細胞内局在

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Entrez Gene: 100364501 Rat
    • Entrez Gene: 100365669 Rat
    • Entrez Gene: 291159 Rat
    • Entrez Gene: 679994 Rat
    • Entrez Gene: 680511 Rat
    • Entrez Gene: 682330 Rat
    • Omim: 602810 Human
    • SwissProt: P68432 Cow
    • SwissProt: P68431 Human
    • SwissProt: P68433 Mouse
    • SwissProt: Q6LED0 Rat
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 397328 Mouse
    • Unigene: 138090 Rat
    see all
  • 別名

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

画像

  • ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)

    Chromatin was prepared from U-2 OS (Human bone osteosarcoma epithelial cell line) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The  ChIP was performed with 25 µg of chromatin, 2 µg of  ab2886 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR. Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886) at 1 µg/ml + Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

    Secondary
    Human NFIB / NF1B2 peptide (ab95051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15.4 kDa
    Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Peptide Array - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    Peptide Array - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)

    All batches of ab2886 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - mono methyl K79 peptide (ab4555), indicating that this antibody specifically recognises the Histone H3 - mono methyl K79 modification.

    ab4558 - Histone H3 - unmodified

    ab1771 - Histone H3 - di methyl K9

    ab4555 - Histone H3 - mono methyl K79

    ab4556 - Histone H3 - di methyl K79

    ab4557 - Histone H3 - tri methyl K79

    ab4560 - Histone H4 - di methyl K79

  • Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    Western blot - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    All lanes : Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886) at 1/300 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Testis (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15.4 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes
  • ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    ChIP - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)This image is a courtesy of Anonymous Abreview

    Chromatin was prepared from whole cell lysate of normal rat liver and liver cancer cells. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 15 minutes in formaldehyde. 5 µg of the primary antibody was used in 1/100 dilution and it was incubated with the sample for 16 hours at 4°C in a commercially available ChIP dilution buffer. The immunoprecipitated DNA was quantified by real time PCR. ChIP results show that the Histone H3 (mono methyl K79) and GAPDH genes are expressed in higher levels in liver cancer cells than in normal liver cells.

    See Abreview

  • Immunoprecipitation - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)
    Immunoprecipitation - Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade (ab2886)

    Histone H3 (mono methyl K79) was immunoprecipitated using 0.5mg HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate, 5µg of Rabbit polyclonal to Histone H3 (mono methyl K79) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2886.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 18kDa: Histone H3 (mono methyl K79).

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

    • Datasheet
  • 参考文献 (47)

    ab2886 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2886 は 47 報の論文で使用されています。

    • Liu C  et al. CBP mediated DOT1L acetylation confers DOT1L stability and promotes cancer metastasis. Theranostics 10:1758-1776 (2020). PubMed: 32042335
    • Nassa G  et al. Inhibition of histone methyltransferase DOT1L silences ERa gene and blocks proliferation of antiestrogen-resistant breast cancer cells. Sci Adv 5:eaav5590 (2019). PubMed: 30775443
    • Chory EJ  et al. Nucleosome Turnover Regulates Histone Methylation Patterns over the Genome. Mol Cell 73:61-72.e3 (2019). PubMed: 30472189
    • Lam KG  et al. Cell-type-specific genomics reveals histone modification dynamics in mammalian meiosis. Nat Commun 10:3821 (2019). PubMed: 31444359
    • Lu X  et al. GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival. Nucleic Acids Res 47:10977-10993 (2019). PubMed: 31612207
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 12 Abreviews or Q&A

    Western blot abreview for Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - nuclear (wildtype, histone H3 mutant, dot1 mutant)
    Gel Running Conditions
    Reduced Denaturing (BioRad AnyKD)
    Loading amount
    1e+006 cells
    Treatment
    UV exposed (200 J/m2)
    Specification
    wildtype, histone H3 mutant, dot1 mutant
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Jeff Thompson

    Verified customer

    投稿 Jul 23 2015

    Western blot abreview for Anti-Histone H3 (mono methyl K79) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    4.5 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - nuclear (whole fruit fly)
    Specification
    whole fruit fly
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mr. Alexander Hambly

    Verified customer

    投稿 Mar 24 2015

    ChIP abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (Hela)
    Specification
    Hela
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Detection step
    Real-time PCR
    Negative control
    No DNA control
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 20 2012

    ChIP abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    ChIP
    Sample
    Mouse Tissue lysate - nuclear (mouse testis)
    Specification
    mouse testis
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Detection step
    Real-time PCR
    Negative control
    No DNA control
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 20 2012

    Western blot abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - nuclear (mouse testis)
    Loading amount
    20 µg
    Specification
    mouse testis
    Gel Running Conditions
    Reduced Denaturing (15%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 20 2012

    Western blot abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Hela)
    Loading amount
    25 µg
    Specification
    Hela
    Gel Running Conditions
    Reduced Denaturing (15%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 20 2012

    ChIP abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Rat Cell lysate - whole cell (Normal Liver and liver cancer cells)
    Specification
    Normal Liver and liver cancer cells
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Detection step
    Real-time PCR
    Positive control
    GAPDH
    Negative control
    Normal IgG
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 17 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Sep 12 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (methylated) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (10T1/2)
    Specification
    10T1/2
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Sep 12 2008

    Question

    Our lab bought several antibodies from your company before. Now I am trying to do ChIP and particularly interested in trying the PCR primers you guys used. I notice that the website of your company does not list this information whereas the delivered information sheets do. I am wondering whether you can help me with the sequences of PCR primers for the following antibodies: ab8895, ab8580, ab2886, ab3594, and ab2621. I appreciate if you can mail me information sheets regarding these A/bs.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 20 2007

    Answer

    All antibodies that are batch tested here at Abcam have the primers that were employed during the testing published as a separate page which can be accessed as a clickable link on the antibody datasheet. For example on the antibody datasheet for ab8895 under "specific protocols" there is a clickable link; "PCR primers used to test ab8895 in ChIP at Abcam" Here you will find all the information necessary to test the antibody. You can locate the same PCR primer information on each of the antibody datasheets you refer to.

    Read More

    Abcam Scientific Support

    Answered on Feb 21 2007

    1-10 of 12 Abreviews or Q&A

    •  戻る
    • 1
    • 2
    • 次へ 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    ブログ

    ブログを読む

    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2021 Abcam plc. All rights reserved.