Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (MDA-MB-231)
Total protein in input
1e+007 cells
Treatment
20ng/ml TNF alpha
Immuno-precipitation step
Other - Protein G Dynabeads
Specification
MDA-MB-231
Other product details
Incubation time
19 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
0.33 µg/10^6 cells
Western Blot antibody
Dilution
1/1000
Name
Additional data
Additional Notes
1ug of antibody to 1A260 of the nuclear lysate was used and incubated for 16hrs at 4 degrees and the matrix was added afterwards and incubated for 3hrs at 4 degrees with rotation. The antibody was poor at IP under ChIP conditions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ifeoluwa Adewumi
Verified customer
投稿 Feb 28 2017