製品の概要

  • 製品名

    Histone H3 Modification Multiplex Assay Kit (Colorimetric)
  • 検出方法

    Colorimetric
  • サンプルの種類

    Tissue, Adherent cells, Suspension cells
  • アッセイタイプ

    Quantitative
  • 検出感度

    0.5 ng/well
  • 検出範囲

    20 ng/well - 500 ng/well
  • 全工程の試験時間

    2h 30m
  • 種交差性

    交差種: Mouse, Rat, Human, Plants, Fungi, Bacteria
  • 製品の概要

    The Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader. 

  • 試験プラットフォーム

    Microplate reader

製品の特性

関連製品

画像

  • Histone extracts were prepared from MCF-7 and MDA-231 cells and multiple Histone H3 modifications were screened and measured using the Histone H3 Modification Multiplex Assay Kit (Colorimetric). 100 ng of total histone proteins were used.

プロトコール

参考文献

This product has been referenced in:

  • Lillico R  et al. HDAC inhibitors induce global changes in histone lysine and arginine methylation and alter expression of lysine demethylases. J Proteomics 133:125-33 (2016). Read more (PubMed: 26721445) »
See 1 Publication for this product

レビューと Q&A

Abreviews
We used this product to detect histone modifications on bovine granulosa cells. It worked really well and gave good
signal. The background was very low even with brief washings.

Protocol:
1. Histone from bovine granulosa cells were extracted using histone extraction kit (ab113476)
2. Protein concentration in lysate was then measured using Bradford assay (BioRad)
3. 50 ng of histone extract was used per well in H3 modification multiplex assay kit (ab185910)

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投稿 Dec 08 2015

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