Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453)
Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell Lysate, Tissue Extracts
製品の概要
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製品名
Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit
Histone Methyltransferase キット 製品一覧 -
サンプルの種類
Tissue Extracts, Cell Lysate -
アッセイタイプ
Quantitative -
全工程の試験時間
3h 00m -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Mammals -
製品の概要
Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. In mammalian cells, histone lysine methyltransferases such as SuVar39h and ESET among others are responsible for methylation of lysine 9 at histone H3.
Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453) allows the user to measure the activity or inhibition of individual histone methyltransferase (HMT) that specifically target histone H3 at lysine 9 (H3K9). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.
ab113453 recognizes methylated H3 at lysine site 9.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 48 tests 96 tests 10X Wash Buffer 1 x 11ml 1 x 22ml 8-Well Assay Strips (with Frame) 6 units 12 units Adomet 1 x 25µl 1 x 50µl Biotinylated Substrate, 25 µg/mL 1 x 100µl 1 x 200µl Capture Antibody, 100 µg/mL 1 x 25µl 1 x 50µl Control Enzyme (300 µg/mL) 1 x 5µl 1 x 10µl Detection Antibody, 200 µg/mL 1 x 10µl 1 x 20µl Developing Solution 1 x 6ml 1 x 12ml Histone Assay Buffer 1 x 1.5ml 1 x 3ml HMT Standard, 10 µg/mL 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml -
研究分野
関連製品
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Related Products
画像
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Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K9 specific histone methyltrasferase activity (G9a) was measured. Purified G9a was spiked into the nuclear extract that has low concentration of nuclear proteins by dilution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab113453 は 2 報の論文で使用されています。
- Srinivasan SP et al. Epigenetic mechanisms of Strip2 in differentiation of pluripotent stem cells. Cell Death Discov 8:447 (2022). PubMed: 36335090
- Ogawa S et al. SETDB1 Inhibits p53-Mediated Apoptosis and Is Required for Formation of Pancreatic Ductal Adenocarcinomas in Mice. Gastroenterology 159:682-696.e13 (2020). PubMed: 32360551