Application
Western blot
Sample
Human Cell lysate - nuclear (Liver)
Loading amount
20 µg
Specification
Liver
Treatment
X protein was knockdowned
Gel Running Conditions
Reduced Denaturing (15)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase-conjugated AffiniPure Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 17 kDa
Positive control
X protein was knockdowned to decrease 2 methylation level of H3K79
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 22 2011