Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human lung endothelial lining)
Specification
Human lung endothelial lining
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 27°C
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500
Additional data
Additional Notes
Requires heat antigen retrieval in citrate buffer. Most of the nuclear markers often require antigen retrieval even though they are frozen sections.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 15 2012