Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (osteosarcoma (U2OS))
Specification
osteosarcoma (U2OS)
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Lab Donkey anti rabbit Cy2
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Additional data
Additional Notes
fixation in 4% PFA for 15 min at RT
permeabilisation for 15 min at RT in 0.5% Triton X 100 in PBS
Figure caption:
Blue: DAPI nuclear staining, Green: Histone H3 (di methyl K4)
Uniformal staining of the whole nucleus, with several specles found. Insert shows H3-di methyl K4 of tw2 cells only
permeabilisation for 15 min at RT in 0.5% Triton X 100 in PBS
Figure caption:
Blue: DAPI nuclear staining, Green: Histone H3 (di methyl K4)
Uniformal staining of the whole nucleus, with several specles found. Insert shows H3-di methyl K4 of tw2 cells only
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Alexander Rapp
Verified customer
投稿 Feb 16 2007