Application
Western blot
Sample
Pig Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (17,5)
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBST + 3% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti rabbit (NEB) 7074S
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL prime
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 15 kDa Non-specific: 0 kDa
Additional data
Additional Notes
https://doi.org/10.1016/j.jnutbio.2019.02.004
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 09 2019