Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa and HEK 293T nuclear lysate)
Gel Running Conditions
Reduced Denaturing (15% SDS gel)
Loading amount
10 µg
Specification
HeLa and HEK 293T nuclear lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1X TBST buffer
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-rabbit IgG-HRP conjugated Santa Cruz
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Thermo Scientific West Pico Chemilumine
Exposure
5 second(s)
Bands
Specific: 15 kDa
Additional data
Additional Notes
The antibody is very powerful. Can be further diluted without loss in signal.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Yupen Chung
Verified customer
投稿 Jan 08 2016