Application
ChIP
Sample
Human Cell lysate - nuclear (Human Embryonic Kidney (HEK293T))
Negative control
Input/Peptide
Specification
Human Embryonic Kidney (HEK293T)
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde (1%)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde (1%)
Positive control
Input/Peptide
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Nuclear Lysis Buffer
Concentration
0.004 µg/µg chromatin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 02 2018