Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Denaturing (15%)
Sample
Arabidopsis thaliana Tissue lysate - nuclear (Seedlings)
Specification
Seedlings
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 23°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: Milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 15 kDa
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Sep 26 2013