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    histone-h3-citrulline-r2--r8--r17-antibody-ab5103.pdf

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Epigenetics and Nuclear Signaling Histones H3 Citrullated
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Anti-Histone H3 (citrulline R2 + R8 + R17) 抗体 (ab5103)

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Reviews (26)Q&A (7)References (352)

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Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
  • Immunocytochemistry - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
  • Immunoprecipitation - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
  • Peptide Array - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)

Key features and details

  • Rabbit polyclonal to Histone H3 (citrulline R2 + R8 + R17)
  • Suitable for: PepArr, IP, ICC, WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (ab281584)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Histone H3 (citrulline R2 + R8 + R17) antibody
    Histone H3 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Histone H3 (citrulline R2 + R8 + R17)
  • 由来種

    Rabbit
  • 特異性

    ab5103 detects a 17 kDa band in single lane Western Blot. Peptide inhibition in Western Blot hasn't been processed. Modification specificity is determined by Peptide Array. ab5103 binds strongly to Histone H3 citrulline 2 + 8 + 17 peptide.
  • アプリケーション

    適用あり: PepArr, IP, ICC, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human, Recombinant fragment
    交差が予測される動物種: Rabbit, Cow, Monkey, a wide range of other species
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab32876)

  • ポジティブ・コントロール

    • WB: Mouse and rat brain tissue lysates. HL60 + DMSO, NIH/3T3 and PC12 whole cell lysates. ICC: MCF7 cells. IP: HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a GFP-tag
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Citrullated

関連製品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Histone H3 (citrulline R2 + R8 + R17) peptide (ab32876)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)
  • Related Products

    • Anti-Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)
    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)
    • Human Histone H3 (tri methyl K4) peptide (ab1342)
    • Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955)
    • Human Histone H3 (tri methyl K9) peptide (ab1773)
    • Human Histone H3 (tri methyl K27) peptide (ab1782)
    • Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)
    • Human Histone H3 (unmodified) peptide (ab7228)
    • Human Histone H3 (di methyl K4) peptide (ab7768)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5103の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
PepArr
Use a concentration of 0.2 - 0.02 µg/ml.
IP
Use at an assay dependent concentration.
ICC
Use a concentration of 1 µg/ml.
WB (9)
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

In our hands, when tested in western blot, this product typically gives a weaker signal in mouse and rat tissue lysates compared to mouse and rat cell lines. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

Abcam recommends using 3-5% milk as the blocking agent

We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.

特記事項
PepArr
Use a concentration of 0.2 - 0.02 µg/ml.
IP
Use at an assay dependent concentration.
ICC
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

In our hands, when tested in western blot, this product typically gives a weaker signal in mouse and rat tissue lysates compared to mouse and rat cell lines. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

Abcam recommends using 3-5% milk as the blocking agent

We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody.

ターゲット情報

  • 機能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 配列類似性

    Belongs to the histone H3 family.
  • 発生段階

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • 翻訳後修飾

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • 細胞内局在

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Entrez Gene: 100364501 Rat
    • Entrez Gene: 100365669 Rat
    • Entrez Gene: 291159 Rat
    • Entrez Gene: 679994 Rat
    • Entrez Gene: 680511 Rat
    • Entrez Gene: 682330 Rat
    • Omim: 602810 Human
    • SwissProt: P68432 Cow
    • SwissProt: P68431 Human
    • SwissProt: P68433 Mouse
    • SwissProt: Q6LED0 Rat
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 397328 Mouse
    • Unigene: 138090 Rat
    see all
  • 別名

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

画像

  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    All lanes : Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking buffer: 2% BSA

    Gel type: MES

  • Immunocytochemistry - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Immunocytochemistry - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)

    Immunocytochemical anaylsis of paraformaldehyde-fixed human HeLa cells, permeabilised in TBST (20 min) and incubated with ab5103 at 1µg/ml for 1h at RT. 1% BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). The nucleus was stained with DAPI (blue).

  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)

    This western blot image is a comparison between ab5103 and the alternative recombinant multiclonal antibody ab281584.

    Left side - Recombinant multiclonal to Histone H3 (citrulline R2 + R8 + R17) - ab281584
    All lanes: Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] ab281584 at 1/1000 dilution.
    Lane 1: Untreated HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a myc-His-tag®, whole cell lysate
    Lane 2: HEK-293T transfected with PADI4 expression vector containing a myc-His-tag® treated with 10 mM calcium chloride and 10 µM lonomycin for 4 hours, whole cell lysate
    Lane 3: Untreated NIH/3T3 (mouse embryonic fibroblast) transfected with PADI4 expression vector containing a myc-His-tag®, whole cell lysate
    Lane 4: NIH/3T3 transfected with PADI4 expression vector containing a myc-His-tag® treated with 10 mM calcium chloride and 10 µM lonomycin for 4 hours, whole cell lysate

    Right side: Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    All lanes: Anti-Histone H3 (citrulline R2 + R8 + R17) antibody ab5103 at 1/1000 dilution.
    Lane 1: Untreated HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a myc-His-tag®, whole cell lysate
    Lane 2: HEK-293T transfected with PADI4 expression vector containing a myc-His-tag® treated with 10 mM calcium chloride and 10 µM lonomycin for 4 hours, whole cell lysate
    Lane 3: Untreated NIH/3T3 (mouse embryonic fibroblast) transfected with PADI4 expression vector containing a myc-His-tag®, whole cell lysate
    Lane 4: NIH/3T3 transfected with PADI4 expression vector containing a myc-His-tag® treated with 10 mM calcium chloride and 10 µM lonomycin for 4 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Why choose a recombinant antibody?
    Research with confidence – consistent and reproducible results with every batch
    Long-term and scalable supply – powered by recombinant technology for fast production
    Success from the first experiment – confirmed specificity through extensive validation
    Ethical standards compliant – production is animal-free

  • Immunoprecipitation - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Immunoprecipitation - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)

    This immunoprecipitation image is a comparison between ab5103 and the alternative recombinant multiclonal antibody ab281584.

    Left side - Recombinant multiclonal to Histone H3 (citrulline R2 + R8 + R17) - ab281584
    All lanes: Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] ab281584 at 1/1000 dilution.
    Lane 1: HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysate 10 μg.
    Lane 2: ab281584 IP in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab281584 in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours whole cell lysate

    Right side: Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    All lanes: Anti-Histone H3 (citrulline R2 + R8 + R17) antibody ab5103 at 1/1000 dilution.
    Lane 1: HEK-293T (human embryonic kidney) transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours, whole cell lysate 10 μg.
    Lane 2: ab5103 IP in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab5103 in HEK-293T transfected with PADI4 expression vector containing a GFP-tag treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours whole cell lysate

    Secondary
    All lanes: VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.


    Predicted band size: 15kDa

    Why choose a recombinant antibody?
    Research with confidence – consistent and reproducible results with every batch
    Long-term and scalable supply – powered by recombinant technology for fast production
    Success from the first experiment – confirmed specificity through extensive validation
    Ethical standards compliant – production is animal-free

  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    All lanes : Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) at 1 µg/ml

    Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) nuclear lysate
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) nuclear lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking buffer: 2% BSA

    Gel type: MES

  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    All lanes : Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) at 0.2 µg/ml

    Lane 1 : HL60 whole cell lysate (negative control)
    Lane 2 : HL60 whole cell lysate + DMSO (solvent control)
    Lane 3 : HL60 whole cell lysate + DMSO + Calcium Ionophore (positive control)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IR680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?



    Loading Control: GAPDH

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab5103 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 secondary at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

     

  • Peptide Array - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Peptide Array - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)

    All batches of ab5103 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - citrulline 2 + 8 + 17 peptide (ab32876), indicating that this antibody specifically recognises the Histone H3 - citrulline 2 + 8 + 17 modifications.

    ab32876 - Histone H3 - citrulline 2 + 8 + 17

    ab17566 - Histone H3 - unmodified

  • Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103)
    All lanes : Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (ab5103) at 1 µg/ml

    Lane 1 : HL60 (Human Caucasian promyelocytic leukaemia) DMSO and Calcium Ionophore treated Whole Cell Lysate with with 5% BSA
    Lane 2 : HL60 (Human Caucasian promyelocytic leukaemia) DMSO and Calcium Ionophore treated Whole Cell Lysate with with 5% milk
    Lane 3 : HL60 (Human Caucasian promyelocytic leukaemia) DMSO and Calcium Ionophore treated Whole Cell Lysate with with 3% milk

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above .

    Blots were developled with Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody

プロトコール

  • ChIP protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (352)

ab5103 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5103 は 352 報の論文で使用されています。

  • Chirivi RGS  et al. Therapeutic ACPA inhibits NET formation: a potential therapy for neutrophil-mediated inflammatory diseases. Cell Mol Immunol 18:1528-1544 (2021). PubMed: 32203195
  • Locke M & Longstaff C Extracellular Histones Inhibit Fibrinolysis through Noncovalent and Covalent Interactions with Fibrin. Thromb Haemost 121:464-476 (2021). PubMed: 33131044
  • Takeuchi M  et al. Neutrophils interact with cholangiocytes to cause cholestatic changes in alcoholic hepatitis. Gut 70:342-356 (2021). PubMed: 33214166
  • Mondal S  et al. Site-specific incorporation of citrulline into proteins in mammalian cells. Nat Commun 12:45 (2021). PubMed: 33398026
  • Shi Y  et al. Neutrophils can promote clotting via FXI and impact clot structure via neutrophil extracellular traps in a distinctive manner in vitro. Sci Rep 11:1718 (2021). PubMed: 33462294
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 33 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (neutrophils/NETs)
Permeabilization
No
Specification
neutrophils/NETs
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Zinc fixative
Read More

DR. Grace Thomas

Verified customer

投稿 Jul 24 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
Yes - Tween 20 in Buffer
Specification
Intestine
Blocking step
(agent) for 5 minute(s) · Concentration: 0.25% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Mr. Mark Butters

Verified customer

投稿 Jul 05 2021

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Mouse brain after stroke)
Antigen retrieval step
None
Permeabilization
No
Specification
Mouse brain after stroke
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 10 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (brain tissue)
Permeabilization
No
Specification
brain tissue
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Ran Xu

Verified customer

投稿 Feb 02 2021

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
Yes - 0.3% triton-PBS
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

正剛 田中 

Verified customer

投稿 Dec 17 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (pulmonary alveolar macrophages and epithelial cell)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6 30min
Permeabilization
No
Specification
pulmonary alveolar macrophages and epithelial cell
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 1.5% · Temperature: 24°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Nov 11 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse neutrophils)
Permeabilization
Yes - 0.2% Triton in PBS
Specification
mouse neutrophils
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Kai Sun

Verified customer

投稿 Nov 10 2020

Western blot abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neutrophils)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
20 µg
Specification
neutrophils
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Aug 05 2020

Western blot abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (Vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Vascular smooth muscle cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 Dec 18 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Vascular smooth muscle cell)
Permeabilization
Yes - NP40
Specification
Vascular smooth muscle cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Dec 18 2018

1-10 of 33 Abreviews or Q&A

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