Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Hela Cells)
Total protein in input
4000 µg
Immuno-precipitation step
Protein G
Specification
Hela Cells
Other product details
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: IP/Lysis Buffer + Protease Inhibitors
Concentration
1 µg/mg lysate
Western Blot antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti H3
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
The IP worked on the Hela cells, but the reason for rating it average is because I had to enrich this mark by introducing mutations.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 31 2011