Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (yeast)
Gel Running Conditions
Non-reduced Denaturing (15)
Loading amount
2e+006 cells
Specification
yeast
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBS-T
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: GE Healthcare ECL Anti-Mouse IgG, HRP linked whole
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Positive control
I lysed in both SUMEB and SUTEB and blotted samples with a rabbit polyclonal anti-H3 primary and anti-rabbit HRP-conjgated secondary and observed the expected H3 bands at ~15 kD. For membranes blotted with ab10799, I re-probed with mouse mono-clonal anti-Beta-tubulin and the GE Healthcare ECL Anti-Mouse IgG, HRP linked whole antibody and observed the expected band at ~50 kD.
Additional data
Additional Notes
Top row:
Lane 1: SUMEB lysis, Lane 2: SUTEB lysis both lanes 1 and 2 probed 1:1,000 with ab10799 and 1:10,000 anti-mouse secondary
Lane 3: SUMEB lysis, Lane 4: SUTEB lysis both lanes 3 and 4 probed 1:5,000 with ab10799 and 1:10,000 anti-mouse secondary
Lane 5: SUMEB lysis, Lane 6: SUTEB lysis both lanes 5 and 6 probed 1:5,000 with rabbit anti-H3 polyclonal and 1:10,000 anti-rabbit secondary
Bottom row:
Membranes with lanes 1-4 were washed and re-probed with 1:1,000 mouse monoclonal anti-Beta tubulin and the same 1:10,000 anti-mouse secondary as that used to detect ab10799 as a positive control for the anti-mouse secondary
Abcam response
Thank you for testing Saccharomyces cerevisiae as part of our AbTrial program. The data suggests that the antibody may not cross react with this species. We appreciate your feedback.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Andrew Lerner
Verified customer
投稿 Sep 25 2015