Application
Western blot
Sample
Human Cell lysate - whole cell (PC-3 (Metastatic Prostate Cancer Cell Line))
Loading amount
15 µg
Specification
PC-3 (Metastatic Prostate Cancer Cell Line)
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Aqua Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 23°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Aqua Block
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse Alexa Fluor 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/5000
Detection
Detection method
Li-Core Fluorescent Reader
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 15 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 10 2011