Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (MDA-MB-231 cells)
Total protein in input
1e+007 cells
Immuno-precipitation step
Other - Protein G Dynabeads
Specification
MDA-MB-231 cells
Other product details
Incubation time
19 hour(s) and 0 minute(s) · Temperature: 4°C
Western Blot antibody
Dilution
1/6000
Name
Abcam antibody:(not specified yet)
Additional data
Additional Notes
Antibody worked really well. The IP was performed under ChIP conditions. in brief, 1ug of antibody to 1A260 of the nuclear lysate was used and incubated for 16hrs at 4 degrees and the matrix was added afterwards and incubated for 3hrs at 4 degrees with rotation. Western blot was run rather than qPCR. ID=immunodepleted.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ifeoluwa Adewumi
Verified customer
投稿 Feb 28 2017