Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (Erythrocytes)
Loading amount
50000 cells
Specification
Erythrocytes
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer diluted 10 times
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IRDye 800CW IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Fluorescence
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~14 kDa
Positive control
Xenopus erythrocytes
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 01 2013